Physical and mechanical properties of rubberwood (Hevea brasiliensis) dyed with Lasiodiplodia theobromae
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Blue staining on rubberwood (Hevea brasiliensis) is a common kind of defect. There currently exists much research focused on the prevention and control of blue staining. However, little research has been concentrated on the utilization of blue staining for green dyeing. The research conveyed in this paper primarily used Lasiodiplodia theobromae to dye rubberwood, and used scanning electron microscope (SEM), energy-dispersive spectrometer (EDS), X-ray diffraction (XRD), and fourier transform infrared spectrometer (FTIR) to analyze the commission internationale eclairage (CIE) L*a*b* value of color, the contact angle, the pH value, 24-h water absorption, mass loss ratio, and compressive strength in increments between 5 and 40 days. The results found that the color of rubberwood became darker and more uniform, and that the surface dyed with fungi can reach a super-hydrophobic state. With the increase of time, the pH value of rubberwood changed from acidic to alkaline. Furthermore, hyphae entered the wood mainly through vessels for their large pore diameter, and reduced water absorption. Mass loss ratio increased gradually between 5 and 40 days. The research in this paper concludes that the microorganism was an effective method of wood dyeing, and lays a foundation for further research.
KeywordsWood dyeing Wood preservation Lasiodiplodia theobromae Rubberwood
scanning electron microscope
fourier transform infrared spectrometer
- L. theobromae
commission internationale eclairage
- Turkey HSD
Tukey’s honestly significant difference
statistical product and service solutions
Coloring of wood is a significant processing technology that can improve surface decoration properties and reduce wood defects, thereby increasing the value of wood . Currently, wood staining primarily depends on physical, chemical and biological methods. Physical method such as thermal modification mainly uses high temperature to induce discoloration. In this process, there also exist chemical changes, for thermal modification can change the content and structure of wood chemical composition, such as cellulose, hemicellulose, lignin, and extractives [2, 3, 4]. However, thermal modification requires significant electrical and thermal energy . The chemical dyeing method mainly uses chemical agents such as chitosan, metal, and pigment dyes to dip or brush the wood, and the dyes either cover the wood surface or enter into the wood cell to change the wood color; however, chemical dyes still have problems with colorfastness, water-fastness, and so on [6, 7, 8]. Furthermore, the chemical method may be harmful to the environment and to human health . Biological method usually utilizes stains to change the color of wood, such as extractives in trees and pigment in microorganism, and that is an effective way on wood staining [10, 11].
Biotechnology is a possible solution for wood processing, and microorganisms have the potential to dye wood. Above all, microorganisms have a close relationship with wood because both are living organisms, and there are many kinds of fungi that live in wood . With the appropriate temperature, humidity, light, and pH, microorganisms can live in wood and change the wood into a variety of colors. However, these discolorations have mostly been regarded as defects of wood [13, 14]. Researchers have proposed a variety of methods to prevent against the discoloration [15, 16, 17]. Wood discoloration is usually caused by decaying fungi, mold, and stain fungi [18, 19]. Mold is unsuitable for utilization in wood dyeing as it is unsafety for human and uncontrollability . Decaying fungi have been investigated for its application for wood decoration, such as in the case of spalting wood . Spalting wood is formed by the metabolic processes of fungi, whereby fungi produce zone lines and pigment inside the wood . In recent years, research on spalting wood mainly concentrated on the screening of fungi, the extraction of pigment, the properties of spalted wood, and production design. The relevant results have indicated that fungal pigments have superior colorfastness and decorative performance [23, 24, 25, 26, 27, 28, 29]. However, decaying fungi decreased the mechanical properties of wood . Compared with decaying fungi, stain fungi do not cause an obvious reduction of mechanical properties . Therefore, stain fungi may have more potential for wood dyeing.
Blue-stained wood is the most prevalent phenomenon in wood stain, and it often takes place on all kinds of wood, and rubberwood (Hevea brasiliensis) is prone to be infested by blue staining fungus. Rubberwood is a tropical hardwood species mainly distributed in countries such as China, Indonesia, Malaysia, Thailand, and India . Rubberwood can be used to make wood-based panels, furniture, and joinery products, but rubberwood is also prone to attack by stain fungi in green and dry conditions. The stain reduces the decorative value of wood, and causes financial losses not only in Southeast Asia, but also in the wood industry worldwide [33, 34]. Much research has classified the blue stain fungi, and some methods have been proposed to prevent wood stain. The results have indicated that the most popular stain fungus is Lasiodiplodia theobromae, which can seriously attack rubberwood in less than 3 days [35, 36, 37, 38, 39]. Additional results have shown that the mechanical properties of stained wood not only did not obviously diminish, but also were slightly enhanced [39, 40, 41, 42]. In recent years, several papers paid attention on the decoration of wood dyed with blue stains [11, 26, 41]. This paper mainly studied the relationship between L. theobromae and rubberwood, sought the path that how the fungi enter the rubberwood, analyzed the degradation of fungi on wood component, and physical and mechanical properties. The purpose was to learn about the mechanism of fungi and rubberwood, control the movement of fungi in rubberwood, and increase the decoration of rubberwood. The final purpose was to control decorative pattern on wood surface in further research.
Materials and methods
Wood and fungi species selection
Lasiodiplodia theobromae is the most popular stain fungi, and rubberwood is easily infested by it; so this paper chose them as experimental material. The rubberwood was provided by Hainan State Farms Forest Industrial Group Co., Ltd. The diameter of the rubberwood was about 20 cm in radial direction, and the wood was cut to the size of 20 × 20 × 20 mm for further use. L. theobromae (Pat.) Griffon & Maubl (cfcc 87131) was provided by the China Forestry Culture Collection Center, and which was extracted from the popular wood in the Siyang, Jiangsu province. According to the Regulations on Biosafety Management of Pathogenic Microorganism Laboratories in China , L. theobromae (Pat.) Griffon & Maubl belongs to the fourth type of pathogenic microorganisms, which have been proven to be safe to human. L. theobromae (Pat.) Griffon & Maubl was generally cultivated into a potato dextrose agar culture medium (PDA) that was obtained from Beijing Aoboxing Biology Technology Co., Ltd.
Expand incubation and inoculation procedure
Lasiodiplodia theobromae (Pat.) placed in PDA slant culture medium were largely cultivated in plain culture medium. The culture dishes were conditioned at (28 ± 1) °C and (80 ± 5)% relative humidity (RH) for 3 days, and hyphae filled with culture dishes.
The steps above were repeated, and the hole puncher was used to make the medium grow for 3 days into the fungi cake, the diameter of which was 5 mm, and then the fungi cake was brought to the middle surface of the medium. The culture dishes were conditioned at (28 ± 1) °C and (80 ± 5)% RH for 7 days. The fungi grew in the culture dish in 3 days, and secreted the pigment over the culture dish in 7 days.
The rubberwood was sterilized in autoclave under 0.1 MPa and 121 °C for 30 min, and then put into a constant temperature humidity chamber for 7 days, which made the moisture content of wood about 12%, and the specific gravity about 0.6–0.65. Next, the wood was placed on the culture in the grain direction. In addition, the wood was cultivated at (28 ± 1) °C and (80 ± 5)% RH for 0, 5, 10, 20, 30, and 40 days, respectively, for the fungi can make the properties of rubberwood change with time increasing. What is more, the number of woods was sufficient, because the number of specimens for most tests in 2.2 was at least 5 times.
Scanning electron microscopy (SEM)
A Gemini SEM 300 made in Germany was used to analyze the distribution of hyphae in wood cells, and the samples were studied under energy-dispersive spectrometer (EDS) analysis to determine the element content of wood. EDS can analysis the element content of sample surface. Before the above steps were taken, the samples were cut into cubes less than 10 mm on each side, and then the top surfaces were coated with gold. The magnification was set from 150 to 500 times enlargement.
X-ray diffraction (XRD) analysis
Fourier transform infrared spectroscopy (FTIR) characterization
FTIR spectra at 0 and 40 days of rubberwood (200 mesh powder) were analyzed with a Nicolet 6700 made in the USA, and with a resolution of 4 cm−1 from 400 to 4000 cm−1. The purpose of this analysis was to observe the differences between the chemical bonds by examining the band values.
Measurement of dyed wood color
The effect of fungi on rubberwood color was analyzed with a NH310 portable colorimeter from Shenzhen 3nh Technology Co., Ltd. L* (lightness and darkness), a* (redness and greenness), and b* (yellowness and blueness) were the color parameters. At first, the colorimeter needs to read the primary L0*, a0*, and b0* values of the untreated wood, and then measure the ΔL*, Δa*, Δb*, and ΔE* values of rubberwood from 5 to 40 days. ΔE* represents the total color difference, and each parameter is the average value of five times.
Color change of rubberwood dyed with fungi was analyzed with a one-way analysis of variance (ANOVA) followed by a Tukey’s honestly significant difference (Turkey HSD) test run on statistical product and service solutions (SPSS) version 18.0, which mainly analyzed the relationship between time and color change.
Measurement of contact angle
The contact angle was measured by a Contact Angle System Optical Contact Angle (DataPhysics, Germany), with time set between 0 and 16 s, and the amount of a drop of water was 3 μL which contacted on cross section. The measurement of each sample was repeated at least 5 times.
Wood pH value characterization
The pH value of rubberwood was tested according to the Chinese standard . The rubberwood was made into powder using 40–60 mesh. A combination of 3 g of wood powder and 30 mL of distilled water was mixed into a 50-mL beaker and blended for 5 min, then remained still for 15 min. It was then blended again for 5 min, and remained still for another 20 min. Finally, the pH value was measured by a pH meter (Sartorius PB-10, Germany) twice.
Method of 24-h water absorption, mass loss ratio, and 24-h swelling of wood
Method of compressive strength parallel to the grain
Results and discussion
EDS analysis of undyed wood, dyed wood at 40 days and fungi
Undyed wood (%)
Dyed wood (%)
According to the bar chart above, relative crystallinity at 0 days was 33.03%, and after fungi cultivated on the rubberwood, the relative crystallinity had a sharp increase to 37.15% at 5 days. The relative crystallinity then decreased gradually between 10 and 40 days, and was measured to be 36.17 and 35.20%, respectively. However, the values of the relative crystallinity of rubberwood dyed with fungi are greater than those of the undyed wood. Although the CaC2O4 crystals produced on wood surface, according to the formula (1), the peaks in the formula were not the peaks in CaC2O4; therefore, it is difficult to explain that the production of CaC2O4 increased the crystallinity in different days. There may be one more possible reason that the reduction of non-crystalline or amorphous regions increased the crystallinity, and the further research is needed.
Effects of fungi pigment on wood color
Wood color dyed with fungi in different days
0 (untreated wood)
− 15.35 ± 0.90
− 0.90 ± 1.37
− 2.53 ± 1.75
15.58 ± 1.19
− 25.15 ± 3.89
0.02 ± 1.73
− 2.35 ± 1.37
25.26 ± 3.13
− 34.24 ± 2.80
− 2.05 ± 0.54
− 4.98 ± 0.56
34.66 ± 2.71
− 39.40 ± 2.05
− 1.69 ± 0.73
− 6.14 ± 1.15
39.91 ± 1.89
− 40.56 ± 1.49
− 0.60 ± 0.33
− 4.19 ± 0.85
40.78 ± 0.18
Effects of fungi on wetting angle
Effects of fungi pigment on pH value of rubberwood
Fungi pigment on pH value of rubberwood from 0 to 40 days
Effects of fungi on mass loss ratio, 24-h water absorption and compressive strength
The ANOVA analysis showed there was no obvious relationship between time and compressive strength (p > 0.05). However, Fig. 6 showed a slight increase at 5 days, and Humar  and Lum et al.  also drew a similar conclusion that the mechanical properties of wood improved after the wood was dyed with fungi, which illustrated mechanical properties would not reduce significantly. The FTIR analysis also showed the relationship between fungi and wood component, which illustrated there were no obviously effects on mechanical properties. What is more, the surface color analysis showed that fungi had the potential to be dyed on the rubberwood.
Lasiodiplodia theobromae can enter the rubberwood through vessels, and control the surface color with time increased. A highly hydrophobic surface formed on the rubberwood dyed with fungi. There was a slight degradation of cellulose and hemicellulose of wood, and the properties of rubberwood were not been affected obviously.
The authors wish to thank the Hainan State Farms Forest Industrial Group Co., Ltd and the China Forestry Culture Collection Center for supporting the experimental materials.
BZ wrote the manuscript, performed the experiment of the study and was responsible for data collection; ZY designed the experiments and analyzed the data. YZ and CQ helped performed the experiment and revised the manuscript. All authors read and approved the final manuscript.
This work was sponsored by the Beijing Outstanding Talent Training Foundation (CN) (2017000020124G092).
The authors declare that they have no competing interests.
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