Study of ss-DNA Adsorption and Nano-mechanical Properties on Mica Substrate with Surface Forces Apparatus
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Many DNA-based devices need to build stable and controllable DNA films on surfaces. However, the most commonly used method of film characterization, namely, the probe-like microscopes which may destroy the sample and substrate. Surface Forces Apparatus (SFA) technique, specializing in surface interaction studies, is introduced to investigate the effects of DNA concentration on the formation of single-stranded DNA (ss-DNA) film. The result demonstrates that 50 ng/μL is the lowest concentration that ss-DNA construct a dense layer on mica. Besides, it is also indicated that at different DNA concentrations, ss-DNA exhibit diverse morphology: lying flat on surface at 50 ng/μL while forming bilayer or cross-link at 100 ng/μL, and these ss-DNA structures are stable enough due to the repeatability even under the load of 15 mN/m. At the same time, an obvious adhesion force is measured: −6.5 mN/m at 50 ng/μL and −5.3 mN/m at 100 ng/μL, respectively, which is attributed to the ion-correlation effect. Moreover, the atomic force microscopy (AFM) images reveal the entire surface is covered with wormlike ss-DNA and the measured surface roughness (1.8±0.2 nm) also matches well with the film thickness by SFA. The desorption behaviors of ss-DNA layer from mica surface occur by adding sodium salt into gap buffer, which is mainly ascribed to the decreased ion-ion correlation force. This paper employing SFA and AFM techniques to characterize the DNA film with flexibility and stable mechanical ability achieved by ion bridging method, is helpful to fabricate the DNA-based devices in nanoscale.
KeywordsSingle-stranded DNA Adsorption Nano-mechanical properties Ion-correlation effect Surface forces apparatus
DNA is a potentially valuable biomaterial since DNA nanotechnology can produce DNA-based thin film on inorganic surface to construct flexible nanostructures with ability of programmable and specific recognition, i.e., selectivity and sensitivity [1, 2]. Based on these film and nanostructure, many DNA-based nanodevices can be fabricated, such as biosensors [3, 4, 5], microfluidic devices , DNA chips , micro arrays [8, 9], disease-diagnosis [8, 9, 10]. The fabrication of these devices depends not only on their chemical and physical properties, but also on the conformation, load-carrying ability and type of DNA. Hence, it is greatly significant to explore the adsorption and nano-mechanical properties of DNA at solid/liquid interface.
The DNA species used in many investigations are long and giant double-stranded DNA (dsDNA) [11, 12, 13], and little attention has been paid to ss-DNA absorption on various substrates. Adamcike et al.  using AFM, showed ss-DNA on mica have compact structures with lumps, loops and super twisting, while on highly oriented pyrolytic graphite (HOPG), adopt a conformation without secondary structures. However, more mechanical properties of ss-DNA adsorbing on solid substrate are expecting further study.
Immobilization of DNA on substrate surface has been investigated by many techniques, such as atomic force microscopy (AFM) [15, 16, 17], scanning tunnel microscope (STM) [18, 19], and scanning electron microscope (SEM) . Among all these ways, AFM is the most commonly used for studying DNA geometry. For instance, Cervantes et al.  compared two methods of binding λ-DNA on mica by AFM, and found distinct DNA networks using deposition buffers, but uniform coverage of isolated λ-DNA in spin-coating due to a particular force. However, the detector with micro-size probe may be inevitable to disrupt sample geometry , affecting the accuracy of DNA characterization. Other instruments can also be used to explore DNA adsorption. Cao et al.  reported an oriented chiral DNA film by SEM, and proposed highly ordered DNA film was aligned by mica crystal with the guiding effect of Mg2+. However, the high-energy electron beam of SEM may locally heat biological sample, and the vacuum environment also limits its application.
In sum, the morphology and dynamic changes of DNA on various substrates have been described by AFM, SEM, STM, but many probe-like microscopes may destroy the geometry of sample and substrate during test. In this paper, a surface forces apparatus (SFA), which specializes in measuring the interaction between two surfaces in solutions, is mainly utilized to discuss the adsorption and load-carrying abilities of ss-DNA on mica. Compared with other techniques, SFA utilizing the sample surface with millimeter-curvature is more ideal to study the adsorption mechanisms of the nano-film at solid/liquid interface. More specifically, it is capable of characterizing the mechanical and adhesive properties of biomaterials confined between two surfaces, and getting the absolute thickness of adsorbed film. Besides, SFA can also describe the density of adsorbed films derived from the refractive index of the medium confined between two surfaces .
Flat crystalline and large-scale substrates are needed in SFA and AFM. Muscovite mica is an ideal inorganic substrate due to its relatively large atomically flat areas and ease of preparation , which is negatively charged in solutions for the release of K+ . Similar to mica, DNA is also negatively charged due to its phosphate backbone , leading to an electrostatic repulsion barrier energy between DNA and mica. Therefore, the substrate requires to be treated in advance in order to tightly fix DNA on mica. Generally, the mica surface is pretreated with divalent or multivalent cations (such as Ni2+, Co2+, spermidine) [11, 13, 26, 27], bridging DNA’s phosphate groups and mica surface. Mica functionalization with silanes is to invert the surface charge to induce DNA binding [17, 28], which may change the DNA conformation and rigidity significantly . In addition, mica surface can also be modified via Langmuir–Blodgett (LB) film technique, to directly measure the interaction between DNA bases .
Modification mica with divalent salts to adsorb DNA is more simple and thus practicable. However, the mechanism of divalent-ion-mediated DNA adsorption is still in dispute. Hansma et al.  initially ascribed that divalent cations penetrate into the “cavities” on mica surface and react with the interfacial hydroxyl groups. However, the subsequent works proposed that DNA adsorption is dominated by the cooperative effect driving condensation with divalent cations, subsequently reacting with surface groups . More recently, there are several researches considering the binding due to the ion-correlation effect [12, 13, 33]. Anyway, the real binding mechanism still requires us to make it clearer. Besides, it is interesting to indicate that some transitional metals cations (Ni2+, Co2+, Zn2+) are effective in DNA adsorption, whereas others (Mn2+, Ca2+, Hg2+) seem not helpful. Apart from ions species, the DNA immobilization and conformation are also influenced by ionic strength , DNA concentration [11, 35] and competition effect among different valence ions [13, 33, 36]. Anyhow, the above-mentioned factors are still not fully understood, and the effects need to be further discussed.
In this paper, we combine the SFA and AFM techniques together to study the adsorption and nano-mechanical properties of ss-DNA molecules on mica surface. Specially, we investigated the effects of ss-DNA concentrations on ss-DNA adsorption behaviors. The results indicate that 50 ng/μl is the lowest concentration that ss-DNA molecules are able to absorb and subsequently form a dense layer on mica. The adsorption structure of ss-DNA was further characterized with AFM. Besides, the desorption of the adsorbed DNA films is also observed by introducing monovalent salts into the gap buffer, which is mainly ascribed to the decreased ion correlation force.
2 Materials and Methods
The ss-DNA of thymine with 80 bases (5′-[TTTT]20-3′), was purchased from Sangon Biotech Co, Ltd. (Shanghai). Cobalt chloride (>98%), sodium chloride (>99.999%) and HEPES (>99.5%) were purchased from Sigma-Aldrich. Muscovite ruby mica of grade 1 was supplied by S&J Trading Inc. Buffer solution was prepared by adding 1 mmol/L CoCl2 or NaCl to 10 mmol/L HEPES buffer, then the mixture was titrated to pH 7.5. All solutions above contain 10 mmol/L HEPES, therefore the buffer solutions discussed below will be called by the cation species. Deionized water was used throughout. All the aqueous solutions were filtered through the Millipore filter with pore size of 0.22 μm (Whatman).
2.2 Surface Preparation
Mica sheets were mechanically cleaved to 3‒5 μm in a laminar cabinet, and cut into pieces of ~1 cm2 with hot Pt wire . Then the mica pieces were sputtered with a reflective silver layer of ~50 nm to generate “fringes of equal chromatic order” (FECO) . Afterwards, two mica sheets were glued (silver down) onto two cylindrical silica disks with epoxy resin (Shell, EPON 1004F), then stored in a vacuum desiccator for use.
The stock of 1000 ng/μL ss-DNA (Thymine) solution (10 mM Tris–HCl, 1 mM EDTA, pH 7.5) was further diluted to the final concentrations (10, 25, 50, 100 ng/μL) with 1 mmol/L CoCl2. A freshly cleaved mica sheet was rinsed with the buffer solution used in ss-DNA dilution. Then, 20 μl diluted ss-DNA solution was dropped on the treated mica surface. After a 20-min incubation, the surface was flushed thoroughly with excessive buffer to remove unbound ss-DNA, and a droplet of buffer was left on mica surface. The DNA-coated disk was kept for use. Finally, two disks were mounted into apparatus in a cross-cylinder geometry. Each experiment was reproducibly repeated twice at least. During each experiment, the hardwall distance measured from three points far apart are nearly the same, implying uniform absorbed DNA layers.
2.3 Instruments—SFA and AFM
The AFM used here is MFP 3D from Asylum Research. AFM images were obtained in tapping mode with silicon tips (spring constant is 2 N/m and tip diameter is 20 nm). The scan rate was typically 1 Hz per line and the modulation amplitude was about a few nanometer. The relative height (surface roughness) of the ss-DNA layers was measured by section analysis.
3 Results and Discussion
3.1 Effect of ss-DNA Concentrations on ss-DNA Adsorption on Mica Surfaces
In the case of 25 ng/μL, the average hardwall is 0.16±0.1 nm, meaning few or no ss-DNA binds to mica within measurement error; As DNA concentration increases to 50 ng/μL, the hardwall distance increases to 1.49±0.2 nm. Considering the measured hardwall distance is equivalent to the dynamic diameter of ss-DNA molecules of 1.2‒1.4 nm in solution , this hardwall presents a dense monolayer composed of ss-DNA molecules flat-lying on mica substrate, as illustrated in Figure 2(b). This is greatly consistent with the previous studies, revealing the formation of close-packed DNA monolayer on mica surface in CoCl2 solution containing various DNA (λ-DNA [12, 13], plasmid DNA [12, 35] or linear DNA ). As DNA concentration further increases to 100 ng/μL, a larger hardwall distance of 2.13±0.2 nm implies an ordered ss-DNA bilayer or a cross-link configuration that DNA molecules cross or overlap each other, which is shown in Figure 2(c). The expected cross-link or condensation configuration of DNA on mica has been reported previously [12, 41], which further identify the above explanation about ss-DNA geometry for DH = 2.13 nm. These adsorptions are tight and stable enough due to the reproducible measurements even beyond 15 mN/m.
Furthermore, Figure 3(c) reveals the roughness of mica surface is less than 0.15 nm, which is smooth enough to be used as a substrate in DNA absorption investigations. However, after ss-DNA absorption illustrated in Figure 3(b), the mica surface becomes uneven and has a high and uniform surface coverage of ss-DNA. To sum up, these AFM results certifies the adsorption morphology and configuration of ss-DNA measured by SFA.
In addition to the difference in configuration, the nano-mechanical properties are also presented in Figure 2(a). An adhesive force between DNA-coated mica and bare mica were measured during surface separation. “Jump in” phenomenon was found only at DNA concentration less than 50 ng/μL and “jump out” in all DNA conditions (note: jump in and jump out phenomenon occur in the case of the interaction force larger than cantilever-spring force when surface approach and separation respectively). Under the condition of less than 25 ng/μL (data of 10 ng/μL not shown), the adhesion reaches −37.5±0.2 mN/m, which is six times higher than those at 50 and 100 ng/μL. However, this value is roughly consistent with the force between two bare micas in the same solution (as discussed below), which further proved few or no ss-DNA absorb on mica at 25 ng/μL. Furthermore, the adhesive force decreases to −6.5±0.2 mN/m (Ead≈−1.38 mJ/m2) at 50 ng/μL and −5.3±0.2 mN/m (Ead≈−1.12 mJ/m2) at 100 ng/μL, which are slightly higher than previous studies , perhaps due to different CoCl2 concentration. These adhesive forces far lower than those between two bare micas actually prove ss-DNA molecules have been assuredly immobilized on mica. Additionally, the adhesive force at 100 ng/μL is slightly less than 50 ng/μL, which was due to the stronger steric effect induced by the more adsorbed ss-DNA.
Generally, the counterion-correlation effect is believed to be the dominant cause of dsDNA adsorption on mica mediated by divalent cations [12, 33]. Therefore, it is also expected that ss-DNA molecules are capable to adsorb onto mica surface immersed in divalent cation solution with the sharing of the ss-DNA and mica counterions. In this case, divalent salt acts as bridge between ss-DNA and mica, thus producing a net attraction that pulls ss-DNA onto mica surface. However, the measured force is greater than that of others who only ascribed adsorption to ion-correlation . This is due to the cooperative effect of divalent metal ions condensation along DNA and their reaction with the surface groups . The electrostatic potentials of DNA induce the increase of surface divalent ions concentration, which promotes the reaction of divalent ions with the surface and further results in the stronger ss-DNA adsorption.
3.2 Desorption of ss-DNA from Mica Surface with Excessive Monovalent Counterion
It is intriguing to note that the measured hardwall changes dramatically in different Steps. The hardwall decreases sharply to ~0.34 nm once the gap buffer was replaced to NaCl (Step 2 in Figure 4). Considering the measurement error, the sharp decrease in hardwall reveals desorption of ss-DNA layer from mica does occur in 1 mmol/L NaCl. Additionally, a long-range repulsion at a distance of 25±2 nm in Step 2 is observed in Figure 4(a), which is likely ascribed to the cooperative combination of double-layer force from electrolyte solution and steric repulsion induced by the released ss-DNA suspended in buffer solution shown in Figure 4(c). In the last step, the hardwall increases to 0.84±0.2 nm, which is far less than that of Step 1. This none-zero hardwall may be due to that the residual ss-DNA in solutions immediately reabsorb onto mica surface once CoCl2 was added, as shown in Figure 4(d).
The desorption strength of ss-DNA is monitored by the binding competition between monovalent and divalent cations on mica surface. Based on Poisson-Boltzmann equation and site-binding model , if monovalent salts were added to the bulk solution containing divalent salts, the divalent and monovalent cations would compete fiercely with each other for the binding sites of mica surface and DNA, thus decreasing markedly the surface density of divalent cations, which directly weakens the binding strength of DNA molecules on mica surface . Therefore, the loosely bound ss-DNA can be easily squeezed out in monovalent salts solution, thus resulting in the desorption of ss-DNA from mica surface.
In addition, the nano-mechanical properties were also measured for studying the desorption behavior of ss-DNA thoroughly. In Step 1, the adhesive force (−5±0.2 mN/m) is consisted with that in Figure 2 within error. However, after the gap buffer was replaced by monovalent salts and returned back to divalent salts, an interesting feature is found in Step 3: the adhesion decreases to half of the original magnitude when the buffer was returned back, which may be due to that the binding sites of mica and DNA sites are occupied by Na+ . All in all, the above phenomenon implies the cobalt ions play a crucial role in binding energy between adsorbed monolayer and bare mica surface.
As we know, due to the competition effect, the decrease in surface concentration of divalent cations by adding monovalent salts will directly weaken the binding affinity of ss-DNA on mica substrate. Meanwhile, high surface density of monovalent cations also inhibits DNA from attracting to mica surface. Each of these is responsible for the reduction of ion-correlation effect, which is believed to be the dominant cause of ss-DNA adsorption. Therefore, the desorption of ss-DNA layer in monovalent salts solution is mainly ascribed to the reduced ion-ion correlation force due to the sharp reduction of Co2+ surface concentration. Furthermore, Na+ in buffer solution may occupy the binding sites on both mica surface and ss-DNA molecules, thus screening the binding sites for Co2+ binding to ss-DNA . In this case, the loosely bound ss-DNA on mica surface can be easily squeezed out under high loads.
The adsorption and desorption behaviors of ss-DNA on mica substrate are studied by SFA technique. The ss-DNA concentrations exploration reveals 50 ng/μL is the lowest concentration for the formation of ss-DNA dense monolayer in divalent solution.
ss-DNA present diverse morphology structures at different concentrations: lying flat on mica at 50 ng/μL, but forming bilayer or cross-link at 100 ng/μL, and these structures are tight and stable enough. An attractive adhesion is measured simultaneously during separation, which is dominated by the correlation effect of divalent cations.
The AFM morphology investigation show the entire surface is covered with condensed wormlike ss-DNA molecules and the measured surface roughness (~1.8±0.2 nm) proves the hardwall of SFA.
The desorption behaviors of ss-DNA from mica occurs during introducing monovalent salts. It is the invalidation of ion-correlation effects due to sharp reduction of Co2+ surface concentration that induces the release of ss-DNA layer from mica. All results presented here can flexibly adjust the thickness of DNA film and improve the stability and mechanical ability of the film, which help to design and fabricate the DNA-based nano-devices in nanoscale.
G-BS, Y-JK and M-HC conceived the research. G-BS and Y-JK designed the experiments. G-BS performed the experiments. G-BS, Y-JK and Y-FC analyzed the data. G-BS and Y-JK wrote the manuscript. All authors read and approved the final manuscript.
Gui-Bin Shen, born in 1991, is currently a master candidate at Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, Southeast University, China and School of Mechanical Engineering, Southeast University, China. He received his bachelor degree from Southwest University of Science and Technology, China, in 2014. His research interests include biomaterial adhesion and the interaction between solid and liquid interface.
Ya-Jing Kan, born in 1985, is currently a lecturer at School of Mechanical Engineering, Southeast University, China. He received his PhD degree from Southeast University, China, in 2015. His research interests include biological adhesion and the interactions between solid and liquid interface.
Min-Hua Chen, born in 1964, is currently an associate professor at School of Mechanical Engineering, Southeast University, China. Her research interests include nano technology and theory.
Yun-Fei Chen, born in 1967, is currently a professor, Cheung Kong Scholar, and PhD candidate supervisor at Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, Southeast University, China and School of Mechanical Engineering, Southeast University, China. His main research interests include micro-nano tribology, heat transfer, fluid dynamics and micro-nano manufacture.
The authors declare that they have no competing interests.
Supported by National Natural Science Foundation of China (Grant No. 51435003), and Jiangsu Provincial Natural Science Foundation of China (Grant No. BK20160670).
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