Transition to annual life history coincides with reduction in cell cycle speed during early cleavage in three independent clades of annual killifish
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Annual killifishes inhabit temporary ponds and their embryos survive the dry season encased in the mud by entering diapause, a process that arrests embryonic development during hostile conditions. Annual killifishes are present within three clades distributed in Africa (one East and one West of the Dahomey gap) and South America. Within each of these phylogenetic clades, a non-annual clade is sister taxon to a annual clade and therefore represent an example of convergent evolution.
Early cleavage of teleost embryos is characterized by a very fast cell cycle (15-30 minutes) and lack of G1 and G2 phases. Here, we decided to investigate rates of early cleavage in annual killifishes. In addition, we specifically tested whether also annual killifish embryos lack G1 and G2 phases.
We used time lapse brightfield microscopy to investigate cell division kinetics during the first developmental stages of annual- and non-annual species belonging to the three different phylogenetic clades. Annual killifishes of all three clades showed cleavage times significantly longer when compared to their non-annual sister taxa (average 35 min vs. average 75 min). Using FUCCI fluorescent imaging of the cell cycle after microinjection in the annual species Nothobranchius furzeri, we demonstrate that the first 5 division are synchronous and do not show a G1 phase. Cell cycle synchronization is lost after the 5th cleavage division.
Our results show, for the first time, that cell cycle rate during cleavage, a trait thought to be rather evolutionary conserved can undergo convergent evolutionary change in response to variations in life-history.
KeywordsNothobranchius Early development Segmentation Life history evolution Midblastula transition Imaging Diapause
Fluorescent ubiquitination-based cell cycle indicator
Life history trade-offs are major forces that shape the evolution of embryonic development. Extreme examples are seasonal species where embryos undergo diapause, a suspension of development to overcome hostile periods. A seasonal life cycle has evolved also in a clade of teleost fishes (suborder Aplocheiloidei) known as annual killifish . Annual killifish inhabit ephemeral bodies of water that fill during the monsoon season and disappear by evaporation after its end. Annual killifish are present in Africa and South America and are adapted to alternating wet and dry seasons. All adult fish die when their habitat dries out and survival of the population is ensured by desiccation-resistant eggs that enter into diapause and remain encased in the dry mud until the next rainy season [2, 3, 4, 5]. Annual killifish, especially those of the genus Nothobranchius, are becoming popular model organisms for studying aging and life-history evolution [3, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16]. Diapause in annual killifish is associated with major metabolic remodeling, where several pathways involved in energy production are modulated in order to minimize the embryo's aerobic metabolism and production of reactive oxygen species allowing quiescence. During diapause, oxygen consumption is suppressed and the cell cycle arrested [4, 17]. In some habitats of annual killifish the duration of the temporary pools is only a few months , and therefore the animals spend the majority of their life in diapause.
The morphogenetic processes of early embryonic development of annual killifish show a remarkable derived character: during epiboly, blastomeres migrate as dispersed cells and remain on the yolk surface for days before they finally re-aggregate to form the embryonic axis . This unique stage of development is often associated with the ability to enter into stasis at diapause I, although this trait varies among species of annual killifish.
During this dispersed state, the blastomeres migrate randomly for days before starting the reaggregation process  (see Additional file 1). After formation of the embryonic axis, the development is arrested at a specific stage of somitogenesis in diapause II  where the embryo can remain for several months (or even years).
Here we investigated the early development (up to the sixth division, cleavage) of annual and non-annual killifish embryos. In the species most closely related to those we study here, Fundulus heteroclitus, it is well described that during the cleavage phase cell divisions are synchronized so that 2, 4, 8, 16 and 32 cells arise in succession. This pattern is broadly conserved in teleost species, and cell cycle during cleavage is extremely fast (in the order of 15 to 30 minutes) in typical model teleosts such as Danio rerio, Oryzia latipes, Gasterosteus aculeatus and also Xenopus laevis. During cleavage there is no transcription of the embryonic genome but only translation of maternal transcripts and the cell cycle lacks the G1 and G2 phases, thus proceeding directly from S- to M-phase [26, 27]. Here, we investigated the rate of early cleavages in annual killifishes embryos to assess whether cell-cycle length evolved differently in annual and non-annual clades.
All the fish used were raised in 35-L tanks at 23 to 25°C. They were fed two to three times a day with frozen Chironomus larvae or living nauplii of Artemia salina, depending on size. Annual killifish were bred in a small 3-L tank with 2 cm of river sand on the bottom for 1 hour. Eggs were collected by sieving the sand with a plastic net. Non-annual killifish were bred by floating an artificial floating bush made of acrylic wool strings (spawning mop) in the tank for 1 hour. Eggs were collected from the mop by hand.
After collection, embryos were sorted and dead eggs and those past the two-cell stage were removed. Chorion projections were removed from three to six of the remaining embryos with forceps (when possible) and embedded in 1.5% SeaPlaque low gelling temperature agarose in a Petri dish. While the agar was still molten, eggs were put on the bottom of the dish and oriented, so that the developing blastodisc (animal pole) was parallel to the dish floor. Time lapse images were captured using a Leica M80 stereomicroscope placed in a temperature-controlled room held at 26°C. Magnification ranged between 25 and 45 times, depending on species. Photos were acquired every two to five minutes, depending on species, with a Nikon Digital Sight DS-Fi1 camera using Nikon Elements F 3.0 software or with a Zeiss AxioCam ERc5s camera using ZEN software. Photos from a single acquisition session were opened with Fiji ImageJ as an image sequence, synchrony between eggs of the same experiment was evaluated and timings at which the different cell divisions occurred were noted.
FUCCI reporter constructs used were previously optimized in for zebrafish . Geminin-azami green and cdt1-kusabira orange were extracted from plasmids pT2KXIG using BamHI and ClaI and sub-cloned in a PcS2 expression vector under the control of an SP6 promoter. Synthetic RNAs were transcibed using mMESSAGE mMACHINE® SP6 Transcription Kit, Ambion: 1 nl of both RNAs (final concentration 400 pg/nl each) was injected intracellularly in one-cell-stage embryos using a Tritech microINJECTOR™. Injected embryos were then embedded in 2% low melting agar in a wilkon dish, oriented and left for 1 hour at 25°C while the agar was hardening.
Fluorescence images were acquired using a Leica TCS SP2 confocal microscope; 250 to 350 μm were scanned and an image was taken every 6 μm (40 to 60 images per embryo in total) for each time point. Acquisitions were performed every 13 minutes for almost 24 hours at room temperature (RT, using 488 and 543 emission lasers. Single images were reconstructed from stacks using Fiji imageJ. All images were edited with GIMP. Colors, contrast, brightness and sharpness were adjusted in order to optimize the contrast of the images. All videos were edited with Sony Vegas™. A sequence of images from a single embryo was chosen, oriented, cropped and saved as a new image sequence. This sequence of images was imported as a continuous video in Sony Vegas™.
Results and discussion
Additional file 2: Annual and non-annual time lapse: comparison between the division rate of annual ( Nothobranchius furzeri ) and non-annual ( Aphyosemion striatum ). Cells divide at the animal pole (top part, above the yolk) and the time at which they cleave is greatly different between the annual and the non-annual species. (WMV 14 MB)
The results of the FUCCI imaging indicate that the slowness of the cell cycle in annual killifish species is not due to the presence of a G1 phase, but rather to a retardation of the S- and M-phases. Further, the first signs of asynchrony are observed already between the fifth and sixth divisions. This is considerably earlier than the tenth division, as originally reported for D rerio in correspondence to the mid blastula transition , and is in line with recent results obtained in O latipes, where the desynchronization is observed between the fifth and the sixth division . In medaka and also in zebrafish, however, the first signs of transcription from the zygotic genome are observed around the sixth division [31, 32] and are defined as pre-MBT transcription. It is possible that in annual fish as well, the activation of the very first zygotic genes corresponds to the first signs of asynchrony.
A striking difference in cell-cycle length between annual and non-annual species was observed in three independent clades of killifish. This datum strongly suggests that transition of life history was the driving force for this phenotypic switch. It is however unclear whether a slow cell cycle provides any evolutionary advantage. Prolongation of cell-cycle in annual killifish could result either from the loss of an evolutionary constraint or from the appearance of a novel evolutionary constraint. In the first scenario, the slow cell-cycle evolved in response to the weakening of a directional selection for rapid development that applies to non-annual killifish, because annual killifish eggs spend months (or even years) before hatching. It is indeed of interest if annualism is the basal trait of all three clades and the non-annual clades lost this basal trait independently. Therefore, in all three in stances, transition to a non-annual lifestyle induced a convergent evolution of fast cell-cycle likely because of re-establishment of a directional selection for developmental speed.
In the second scenario, cell cycle is extended as a result of a specific adaptation in order to prolong a period when the embryo can respond with phenotypic plasticity to environmental conditions. In fact, annual killifish embryos can skip diapause when environmental conditions are favorable . Diapause skipping can be an advantage if the water body fills more than once during a season  allowing a second generation and the commitment to this alternative developmental pathway is determined during the early development . So, slowing the cleavage phase would prolong the amount of time available to embryos for responding to environmental conditions.
Our results show, for the first time, that cell-cycle rate during cleavage, a trait thought to be rather evolutionary conserved can undergo convergent evolutionary change in response to variations in life history.
The experiments did not cause any distress to the animals. The protocols of fish maintenance were authorized by the Italian Ministry of Health (Authorization number 96/2003-A).
We wish to thank Roberto Barbuti for hospitality in the Museo di Storia Naturale e del Territorio di Calci and A Miyawaki for providing the FUCCI plasmids. This work was partially supported by internal grant of SNS.
Additional file 3: Fluorescent ubiquitination-based cell cycle indicator (FUCCI) time lapse. Cell cycle progression is visualized by FUCCI. Green cells are dividing cells in the S-/G2 phase, red cells are G1-phase cells; most of the fluorescent protein accumulates in the nuclei. Cells divide synchronously (appear and disappear together) until 32 cells are reached, after which asynchrony starts and cells start to cycle and divide at different times (different colors of cells at the same time, and different timing of appearing/disappearing). (MP4 9 MB)
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