Relationship between smoking and a new index of arterial stiffness, the cardio-ankle vascular index, in male workers: a cross-sectional study
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Cigarette smoking is one of the major factors that increases arterial stiffness. The purpose of this study was to examine further the relationship between smoking status and arterial stiffness using a new index, the cardio-ankle vascular index (CAVI), in male Japanese workers.
This cross-sectional study included 4,729 male Japanese workers undergoing annual health checkups. CAVI was measured at the time of the annual health checkup between April 2007 and March 2008. The subjects were divided into three groups, smokers (n = 1,913), former smokers (n = 1,481) and non-smokers (n = 1,348) according to their responses to a questionnaire. We compared the CAVI in the three groups after adjusting for age. Multiple regression analysis was used to examine the association between CAVI and the number of cigarettes smoked per day in order to examine whether there was a dose–response relationship between smoking and CAVI.
The mean CAVI for each group was 7.81 ± 0.02 for smokers, 7.70 ± 0.02 for former smokers and 7.64 ± 0.02 for non-smokers. A significant difference was observed between each group. According to the results of multiple regression analysis, the standardized β of the number of cigarettes smoked per day was 0.09 (p < 0.01). This confirmed a positive association with CAVI.
Our study demonstrated that there is a significant association between the number of cigarettes smoked per day and arterial stiffness, as measured by CAVI.
KeywordsCardio-ankle vascular index Smoking Arterial stiffness Cardiovascular disease
Cardiovascular disease is a major cause of death in industrialized countries . In Japan, too, the rate of cardiovascular disease as a cause of morbidity has noticeably increased with the westernization of lifestyles. Arterial stiffening is a major factor in cardiovascular disease because of the reduced capacity in blood vessels and the concomitant rise in pulse pressure and fall in shear stress . Therefore, assessment of arterial stiffness is believed to be useful in the prevention of cardiovascular disease.
Non-invasive indices of arteriosclerosis, especially brachio-ankle pulse wave velocity (baPWV), are commonly used for measuring arterial stiffness. An association has been demonstrated between baPWV values and arteriosclerotic diseases, including cardiovascular disease . However, baPWV is strongly dependent on the subject’s blood pressure (BP) at the time of measurement and it is sometimes difficult to assess the baPWV value during treatment for hypertension or as a result of ‘white coat‘ hypertension. Recently, the cardio-ankle vascular index (CAVI), a new convenient method of non-invasive measurement, has been introduced to assess arterial stiffness. CAVI was developed by measuring PWV from the heart to the ankle, as well as BP . CAVI represents arterial stiffness, specifically the elastic properties of the arterial wall between the aortic arch and distal arteries of the lower extremities. Several reports have shown that CAVI is associated with atherosclerotic disease and particularly reflects arteriosclerosis of the aorta, femoral and tibial arteries [4, 5, 6]. One of CAVI’s greatest merits is that measurements remain essentially unaffected by BP, in contrast to other non-invasive parameters of arterial stiffness .
Some studies in Japan suggested that chronic smoking affects arterial stiffness [8, 9]. Cigarette smoking induces changes in both peripheral and central vascular function, even in young or middle aged smokers . However, there are few large-scale studies on the relationship between smoking status and arterial stiffness in subjects of working age. In this cross-sectional study, we examined whether there was an association between smoking status and arterial stiffness, as measured by CAVI, and whether there was a dose–response relationship between smoking and CAVI.
Subjects and methods
Study subjects were participants in annual health examinations conducted between April 2007 and March 2008 by a Japanese electrical machinery manufacturing company who requested a CAVI assessment of arterial stiffness. CAVI examinations were performed in 4,831 male workers in a fasting state. Workers whose CAVI values were taken after a meal (n = 5) or were unreliable (six with ankle brachial index (ABI) <0.9, 65 with arrhythmia ) or could only be measured on one side (n = 23), as well as any workers who declined to provide information on their smoking status (n = 3), were excluded from our study. Finally, a total of 4,729 subjects were selected. Written informed consent was obtained from each examinee regarding the use of his data for research purposes prior to the study. This study was approved by the ethics committee of Hitachi General Hospital.
Cardio-ankle vascular index
where Ps is systolic BP (SBP); Pd is diastolic BP (DBP);ΔP is SBP-DBP;ρis blood density; and PWV is pulse wave velocity. PWV was measured from the aortic valve to the ankle. Detailed CAVI measurement methods and principles have been previously reported . All these measurements and calculations were performed automatically. The mean bilateral CAVI was used in our analysis. ABI was measured at the same time.
Height and weight were measured using an automated scale (Tanita BF-220, Tokyo, Japan) with the participants wearing a light gown. Body mass index (BMI) was calculated as the weight in kilograms divided by the square of height in meters. Other laboratory data obtained were glycosylated hemoglobin (HbA1c), blood sugar, total cholesterol, low-density lipoprotein (LDL) cholesterol, high-density lipoprotein (HDL) cholesterol and triglycerides in the fasting state.
Blood pressure measurement
BP was measured by trained nurses with participants in the sitting position after sufficient rest. Pulse rate was measured at the same time. If SBP was over 140 mmHg or DBP was over 90 mmHg, BP was measured again. We used the ES-H55 (Terumo, Tokyo, Japan) to measure BP.
A self-administered questionnaire was used to collect information on smoking status (smoker, former smoker, non-smoker), the average number of cigarettes smoked per day (or previously smoked for former smokers), history of treatment for lifestyle diseases (diabetes mellitus, high BP, dyslipidemia) and average alcohol consumption per week. Average alcohol consumption per week was assessed by “go” in this study. A “go” is a traditional Japanese unit consisting of 23 g ethanol.
Statistical analysis was performed using SPSS Version 15 for Windows (SPSS, Chicago, USA). Continuous and categorical data are expressed as mean ± standard deviation (SD) and number (percentage), respectively. A p-value < 0.05 was considered statistically significant. Baseline characteristics of the participants were compared by Tukey’s t-test. We compared the mean CAVI among the three groups: smokers, former smokers and non-smokers. However, non-invasive parameters of arterial stiffness, including CAVI, are strongly dependent on age. Therefore, data were compared as mean ± SD adjusted for age by analysis of covariance (ANCOVA). In addition, multiple stepwise regression analysis was performed to examine the relation between average number of cigarettes smoked per day and CAVI. Dependent variables were 1) age; 2) BMI; 3) average number of cigarettes smoked per day; 4) SBP; 5) DBP; 6) pulse rate; 7) LDL cholesterol; 8) HDL cholesterol; 9) triglycerides; 10) total cholesterol; 11) HbA1c; 12) alcohol consumption (the amount of “go” consumed per week).
Baseline characteristics of 4,729 male Japanese workers in this study between April 2007 and March 2008, according to Smoking Status
Non-smoker (n = 1,343)
Former smoker (n = 1,479)
Smoker (n = 1,907)
48.5 ± 8.6
51.6 ± 7.5*
48.7 ± 8.1
Body Mass Index
24.3 ± 3.1*
24.3 ± 2.8*
24.0 ± 3.2
Blood pressure (mmHg)
121.5 ± 11.5*
122.7 ± 11.1*
118.9 ± 12.1
77.8 ± 8.0*
78.8 ± 7.6*
76.1 ± 8.3
Pulse rate (per minute)
68.9 ± 10.0
69.0 ± 9.8
68.2 ± 9.1
Fasting serum cholesterol (mg/dl)
126.0 ± 27.7
125.6 ± 27.7
124.6 ± 30.9
57.0 ± 13.7*
56.9 ± 14.3*
52.6 ± 13.1
112.3 ± 1.6*
118.1 ± 1.7*
129.2 ± 1.7
104.3 ± 17.0
108.3 ± 22.7*
105.5 ± 21.5
Hemoglobin A1c (%)
5.4 ± 0.6*
5.5 ± 0.8
5.5 ± 0.8
Alcohol consumption (“go”/week)
3.86 ± 4.7*
6.11 ± 5.5*
5.91 ± 5.9
Number of cigarettes smoked per day
19.4 ± 8.5
19.0 ± 6.5
Under medical treatment (n (%))
High blood pressure
150 (11.2 %)
269 (18.2 %)*
209 (11 %)
86 (6.4 %)
155 (10.5 %)*
125 (6.6 %)
43 (3.2 %)*
95 (6.4 %)
95 (5 %)
Results of multiple linear regression analysis conducted to assess the relationship of CAVI to measures of variables in non-smoking group and smoking group (n = 3,250)
95%CI of β
Age (actual age)
Number of cigarettes smoked per day (actual number)
Adjusted R square
The study indicated that CAVI, the new index of arterial stiffness, differs significantly after adjustment for age in Japanese male workers, depending on their smoking status. Many studies have reported that smoking status affects the non-invasive index of arterial stiffness or arteriosclerosis [8, 9]. In addition, previous studies showed that the average number of cigarettes smoked per day influenced arterial stiffness . We were able to confirm these relationships by using CAVI, which is the new index of arterial stiffness.
Various studies have also shown that smoking plays a major role in the occurrence of arteriosclerosis and arteriosclerotic disease [12, 13, 14, 15, 16]. Smoking promotes arteriosclerosis because it induces nitric oxide damage to vascular endothelial cells, contributes to high BP or diabetes mellitus, and stimulates coagulation and fibrinolysis pathways . Arterial stiffness is thought to be associated with arteriosclerosis. However, it is ethically difficult to measure arteriosclerosis by invasive methods. CAVI measures arterial stiffness noninvasively and conveniently, and this advantage makes CAVI an appropriate method for assessment of arteriosclerosis caused by smoking in working people.
The CAVI of former smokers was lower than that of smokers but higher than that of non-smokers. Smoking cessation would not result in an immediate restoration of CAVI to non-smoker levels. It was shown that it took 10–14 years after smoking cessation for the majority of benefits to occur. There was no significant difference between the mortality rates of non-smokers and former smokers 10–14 years after smoking cessation . Similarly, most other improvements in CAVI as a result of smoking cessation may take a long time.
The Brinkman index (the number of cigarettes smoked per day multiplied by the number of years of smoking) is commonly used to estimate the cumulative dose of smoking. In this study, however, we thought age would impact the Brinkman index and, therefore, we used the number of cigarettes smoked per day for our analysis.
Our study has several limitations. First, it is a cross-sectional study. A cohort study is required to accurately assess the causal correlation. We will continue to measure the subjects’ CAVI and obtain information on their smoking status at annual health checkups. Another limitation was the fact that all study subjects were male workers and thus our findings cannot be applied to women. There may be differences between men and women in CAVI due to lifestyle or disease and therefore it is necessary to conduct studies that include female subjects to determine any distinction between the genders.
Our study showed an association between smoking status and CAVI in Japanese male workers. CAVI was higher in smokers than in non-smokers and ex-smokers adjusted for age. In addition, the number of cigarettes smoked per day is related to the degree of arterial stiffness, as measured by CAVI.
This work was supported, in part, by Sompo Japan Research Institute Inc.
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