Genetics Selection Evolution

, 35:573 | Cite as

Molecular evolution of the leptin exon 3 in some species of the family Canidae

  • Agata Chmurzynska
  • Magdalena Zajac
  • Marek SwitonskiEmail author
Open Access


The structure of the leptin gene seems to be well conserved. The polymorphism of this gene in four species belonging to the Canidae family (the dog (Canis familiaris) – 16 different breeds, the Chinese racoon dog (Nyctereutes procyonoides procyonoides), the red fox (Vulpes vulpes) and the arctic fox (Alopex lagopus)) were studied with the use of single strand conformation polymorphism (SSCP), restriction fragment length polymorphism (RFLP) and DNA sequencing techniques. For exon 2, all species presented the same SSCP pattern, while in exon 3 some differences were found. DNA sequencing of exon 3 revealed the presence of six nucleotide substitutions, differentiating the studied species. Three of them cause amino acid substitutions as well. For all dog breeds studied, SSCP patterns were identical.


leptin Canidae polymorphism 

(To access the full article, please see PDF)

Copyright information

© INRA, EDP Sciences 2003

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.

Authors and Affiliations

  • Agata Chmurzynska
    • 1
  • Magdalena Zajac
    • 1
    • 2
  • Marek Switonski
    • 1
    Email author
  1. 1.Department of Genetics and Animal BreedingAugust Cieszkowski Agricultural University of PoznanPoland
  2. 2.Institute of Human GeneticsPolish Academy of SciencesPoznanPoland

Personalised recommendations