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High Level of APOA1 in Blood and Maternal Fetal Interface Is Associated With Early Miscarriage

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Abstract

Early miscarriage (EM) is one of the most devastating obstetrical complications globally affecting the quality of women’s life. In the present study, we aimed to identify proteins that correlate with and could act as biomarkers for EM. We performed 2-dimensional gel electrophoresis in chorionic villi samples followed by mass spectrometry for identification of differential protein expression with EM. Proteomic studies detected a total 124 protein spots, out of which 83 spots were differentially expressed between EM and controls in chorionic villi samples. Matrix assisted laser desorbtion/ionization-time of flight (MALDI-TOF) mass spectrometry analysis revealed Apolipoprotein A1 (APOA1) to be the most upregulated protein in the EM group that was validated by Western blotting and Enzyme-linked immunosorbent assay (ELISA) . We found low but not statistically significant level of APOA1 on 21st day of menstruation in comparison to the 7th day. APOA1 level was observed to be the lowest in the first trimester. Hence, this study suggests that low APOA1 expression is critical in establishing pregnancy and elevated APOA1 expression in chorionic villi correlates with EM. Similar observation in serum samples suggests its potential as a marker for the risk of EM.

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Authors and Affiliations

  1. Department of Molecular & Human Genetics, Banaras Hindu University, Varanasi, Uttar Pradesh, 221005, India

    Priyanka Verma MTech, Suchita Singh PhD & Kiran Singh PhD

  2. Department of Molecular & Human Genetics, Weizmann Institute of Science, Rehovot, Israel

    Rohini R. Nair PhD

  3. Division of Endocrinology, CSIR-CDRI, Lucknow, Uttar Pradesh, India

    Singh Rajender PhD & Rajesh K. Jha PhD

  4. Department of Obstetrics & Gynecology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India

    Anuradha Khanna MD

Authors
  1. Priyanka Verma MTech
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  2. Rohini R. Nair PhD
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  3. Suchita Singh PhD
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  4. Singh Rajender PhD
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  5. Anuradha Khanna MD
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  6. Rajesh K. Jha PhD
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  7. Kiran Singh PhD
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Correspondence to Kiran Singh PhD.

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Authors’ Note

The human related work was done at the Department of Molecular & Human Genetics, Banaras Hindu University, Varanasi 21005, India. Priyanka Verma and Rohini R. Nair contributed equally to this work. R.R.N. performed and analyzed the experiment shown in Figure 1. P.V. performed and analyzed the experiments shown in Figure 2. A.K. provided the human tissue and blood samples. S.S. and S.R. helped in the preparation of manuscript. K.S. and R.K.J. designed and coordinated the study design and finalized the manuscript. All authors reviewed the results and approved the final version of the manuscript. Priyanka Verma and Rohini R.Nair are contributing equally for the manuscript that has been mentioned in the original version.

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Verma, P., Nair, R.R., Singh, S. et al. High Level of APOA1 in Blood and Maternal Fetal Interface Is Associated With Early Miscarriage. Reprod. Sci. 26, 649–656 (2019). https://doi.org/10.1177/1933719118783266

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