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The Hyaluronic Acid System is Intact in Menstrual Endometrial Cells in Women With and Without Endometriosis

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Abstract

Objective

To characterize the production and degradation of hyaluronic acid (HA) in menstrual endometrial epithelial cells (EECs) and endometrial stromal cells (ESCs) in women with and without endometriosis. To identify the presence of CD44, the primary receptor of HA, in menstrual EECs and ESCs in women with and without endometriosis.

Design

In vitro study.

Setting

Academic center.

Patient(s)

Deidentified patient samples from women with and without endometriosis.

Interventions

EECs and ESCs were isolated from menstrual endometrial biopsies performed on women with (N = 9) and without (N = 11) endometriosis confirmed by laparoscopy. Main Outcome Measure: Real-time polymerase chain reaction, Western blot, and immunohistochemistry were used to assess hyaluronic acid synthase (HAS) isoforms 1, 2, and 3; hyaluronidase (HYAL) isoforms 1 and 2; and standard CD44. Student t test was used to analyze the results.

Results

There was no significant difference in messenger RNA (mRNA) or protein expression of HAS2, HAS3, HYAL1, or HYAL2 in EECs or ESCs from women with or without endometriosis. HAS1 mRNA was variably detected, whereas HAS1 protein was similarly expressed in EECs and ESCs from women with and without endometriosis. Standard CD44 was expressed in both cell types, and expression did not differ in cells from women with or without endometriosis.

Conclusions

The HA system is expressed in eutopic menstrual ESCs and EECs from women with and without endometriosis. There are no differences in expression in HA production or degradation enzymes in EECs or ESCs from women with and without endometriosis. Standard CD44 expression does not differ in eutopic menstrual endometrial cells from women with and without endometriosis.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Dr, MC 7836, San Antonio, TX, 78229, USA

    Jennifer F. Knudtson MD, Jessica E. McLaughlin MD, Marlen Tellez Santos BS, MS, Peter A. Binkley BS, Rajeshwar R. Tekmal PhD & Robert S. Schenken MD

Authors
  1. Jennifer F. Knudtson MD
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  2. Jessica E. McLaughlin MD
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  3. Marlen Tellez Santos BS, MS
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  4. Peter A. Binkley BS
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  5. Rajeshwar R. Tekmal PhD
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  6. Robert S. Schenken MD
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Corresponding author

Correspondence to Jennifer F. Knudtson MD.

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Knudtson, J.F., McLaughlin, J.E., Santos, M.T. et al. The Hyaluronic Acid System is Intact in Menstrual Endometrial Cells in Women With and Without Endometriosis. Reprod. Sci. 26, 109–113 (2019). https://doi.org/10.1177/1933719118766257

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  • DOI: https://doi.org/10.1177/1933719118766257

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