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Prostaglandin E Pathway in Uterine Tissue During Window of Preimplantation in Female Mice Mated With Intact and Seminal Vesicle-Excised Male

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Abstract

Prostaglandin E2 (PGE2) has been introduced as an important factor for embryo implantation. So the present study was carried out to evaluate the effect of seminal fluid (SF) on PGE2 pathway in uterus tissues of mice during window of preimplantation. The messenger RNA (mRNA) expressions of microsomal PGE synthase (mPGES) and cytosolic PGE synthase (cPGES) as well as protein expression of PGE receptor 2 and 4 (EP2 and EP4) were determined in uterine tissue of control and seminal vesicle-excised (SVX)-mated female mice during days I to 5 of pregnancy using real-time polymerase chain reaction (qRT-PCR) and Western blotting, respectively. We found that mRNA expression of mPGES at day I and 2 of pregnancy was significantly higher in the control group than the SVX-mated group (P <.05), but such result was not obtained for cPGES expression. The protein levels of EP2 at day I to 4 of pregnancy were significantly higher in the control group compared with the SVX-mated group (P <.05), also the EP4 levels were significantly different between the control and SVX-mated groups at the first day of pregnancy (P <.05). Implantation rate was higher in the control group and also there were positive correlations between mPGES and EP2 expressions in the fifth day of pregnancy with implantation rate. Our results demonstrated significant effect of SF on uterine expressions of the evaluated factors, especially mPGES and EP2. Regarding the correlations between levels of these factors and implantation rate, we suggest that possibly one of the important mechanisms of SF in affecting female pregnancy is through mPGES and EP2.

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Authors and Affiliations

  1. Women’s Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Mahnaz Shahnazi MSc, Mohammad Nouri PhD, Zeinab Latifi PhD & Mustafa Mohammadi PhD

  2. Department of Physiology, Faculty of Medicine, Women’s Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Mahnaz Shahnazi MSc & Mustafa Mohammadi PhD

  3. Department of Reproductive Biology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran

    Mohammad Nouri PhD & Amir Fattahi PhD

  4. Neuroscience Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Gisou Mohaddes PhD

Authors
  1. Mahnaz Shahnazi MSc
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  2. Mohammad Nouri PhD
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  3. Gisou Mohaddes PhD
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  4. Zeinab Latifi PhD
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  6. Mustafa Mohammadi PhD
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Correspondence to Mustafa Mohammadi PhD.

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Shahnazi, M., Nouri, M., Mohaddes, G. et al. Prostaglandin E Pathway in Uterine Tissue During Window of Preimplantation in Female Mice Mated With Intact and Seminal Vesicle-Excised Male. Reprod. Sci. 25, 550–558 (2018). https://doi.org/10.1177/1933719117718272

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  • DOI: https://doi.org/10.1177/1933719117718272

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