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The Role of MGAT5 in Human Umbilical Vein Endothelial Cells: Possible Relevance to the Pathological Mechanism of Preeclampsia

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Abstract

Preeclampsia (PE) is associated with shallow invasion of the trophoblast and insufficient remodeling of the uterine spiral artery. Glycosylation reactions are catalyzed by glycosyltransferases including N-acetylglucosaminyltransferase V (MGAT5) and accumulating evidence suggests that MGAT5 is correlated with the migration, proliferation, and survival of various cell types. Our previous study confirmed that MGAT5 is a negative regulator of trophoblast migration and invasion via the direct or indirect inhibition of matrix metalloproteinase 2/9 activity. The primary purpose of this study is to investigate the role of MGAT5 in the function of human umbilical vein endothelial cells (HUVECs) during the development of PE. We observed that MGAT5 was specifically localized within the decidual cells and endothelial cells in maternal decidual tissues. The expression of MGAT5 was elevated in PE placentas compared with the normal control placentas. Moreover, the expression of MGAT5 was increased in hypoxia-reoxygenation (H/R)-treated HUVECs. The knockdown of MGAT5 and PD98059 treatment significantly enhanced cell migration in vitro, promoted tube formation capacity, and inhibited apoptosis in H/R-exposed HUVECs. Our data suggest that oxidative stress induces the overexpression of MGAT5 via the regulation of the focal adhesion kinase-extracellular signalregulated kinase signaling pathway, which, in turn, affects the function of endothelial cells, which then participates in the pathogenesis of PE.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, The First Affiliated Hospital of Chongqing Medical University, No. 1 Youyi Road, Yuzhong District, Chongqing, 400016, China

    Qinyin Deng MD, Ying Chen MD, Nanlin Yin MD, Nan Shan MD, Xin Luo MD, Yu Yuan MD, Xiaofang Luo MD, Yangming Liu MD, Xiru Liu MD & Hongbo Qi MD, PhD

  2. China-Canada-New Zealand Joint Laboratory of Maternal and Fetal Medicine, Chongqing Medical University, No. 1 Youyi Road, Yuzhong District, Chongqing, 400016, China

    Qinyin Deng MD, Ying Chen MD, Nanlin Yin MD, Nan Shan MD, Xin Luo MD, Yu Yuan MD, Xiaofang Luo MD, Yangming Liu MD, Xiru Liu MD & Hongbo Qi MD, PhD

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  1. Qinyin Deng MD
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  2. Ying Chen MD
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  3. Nanlin Yin MD
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  4. Nan Shan MD
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  5. Xin Luo MD
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  6. Yu Yuan MD
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  7. Xiaofang Luo MD
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  8. Yangming Liu MD
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  9. Xiru Liu MD
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  10. Hongbo Qi MD, PhD
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Deng, Q., Chen, Y., Yin, N. et al. The Role of MGAT5 in Human Umbilical Vein Endothelial Cells: Possible Relevance to the Pathological Mechanism of Preeclampsia. Reprod. Sci. 24, 313–323 (2017). https://doi.org/10.1177/1933719116655602

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