Abstract
Objective
To compare uterine artery pulsatility index (PI) and 3-dimensional (3D) placental volume values in first-trimester pregnancies conceived naturally or through in vitro fertilization (IVF) technique using either fresh or cryopreserved embryo and to relate these measurements with preeclampsia (PE) development.
Methods
Uterine artery PI and placental volume were measured at 11 + 0 to 13 + 6 weeks of gestation in 266 IVF pregnancies (139 women with fresh embryo and 127 women with frozen-thawed embryo transfer) and in 266 spontaneously conceived pregnancies matched for maternal age. Nulliparous women with singleton pregnancies were recruited. The mean, highest, and lowest uterine artery PI and placental volume values measured were converted to multiples of the expected normal median (MoM) adjusted for gestational age. The MoM values of IVF pregnancies were compared with those of the naturally conceived group and related to PE development.
Results
No differences were found in uterine artery PI MoM between the 3 groups. Placental volume was significantly lower than in both IVF groups when compared to the controls (fresh embryo IVF Z = 9.33; P ≤.0001; frozen-thawed embryos IVF Z = 3.1; P =.04). The IVF pregnancies with fresh embryos showed placental volume MoM values significantly lower than in the frozen-thawed embryo IVF pregnancies (U = 5.4; enP ≤.0001). In fresh embryo IVF pregnancies developing PE placental volume values resulted lower than in the normotensive ones (U = 2.11; P =.03), while no differences were found for uterine artery PI values.
Conclusion
Firsttrimester placental volume, as assessed by 3D ultrasound, is reduced in IVF pregnancies, and these differences are more marked in those obtained with fresh embryos than those obtained with cryopreservation. This may explain the better obstetrical and perinatal outcomes occurring with the former technique.
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References
Helmerhorst, FM, Perquin, DA, Donker, D, Keirse, MJ Perinatal outcome of singletons and twins after assisted conception: a systematic review of controlled studies. BMJ. 2004;328(7434): 261.
Jackson, RA, Gibson, KA, Wu, YW, Croughan, MS Perinatal out-comes in singletons following in vitro fertilization: a meta- anal-ysis. Obstet Gynecol. 2004;103(3):551–563.
McDonald, SD, Han, Z, Mulla, S, Murphy, KE, Beyene, J, Ohlsson, A, on behalf of the Knowledge Synthesis Group. Preterm birth and low birth weight among in vitro fertilization singletons: a systematic review and meta-analyses. Eur J Obstet Gynecol Reprod Biol. 2009;146(2):138–148.
Maheshwari, A, Pandey, S, Shetty, A, Hamilton, M, Bhattacharya, S Obstetric and perinatal outcomes in singleton pregnancies result-ing from the transfer of frozen thawed versus fresh embryos gen-erated through in vitro fertilization treatment: a systematic review and meta-analysis. Fertil Steril. 2012;98(2):368–377.
Shapiro, BS, Daneshmand, ST, Garner, FC, Aguirre, M, Hudson, C, Thomas, S Evidence of impaired endometrial receptivity after ovarian stimulation for in vitro fertilization: a prospective rando-mized trial comparing fresh and frozen-thawed embryo transfer in normal responders. Fertil Steril. 2011;96(2):344–348.
Papanikolaou, EG, Bourgain, C, Kolibianakis, E, Tournaye, H, Devroey, P Steroid receptor expression in late follicular phase endo-metrium in GnRH antagonist IVF cycles is already altered, indicating initiation of early luteal phase transformation in the absence of secretory changes. Hum Reprod. 2005;20(6): 1541–1547.
Horcajadas, JA, Diaz-Gimeno, P, Pellicer, A, Simon, C Uterine receptivity and the ramifications of ovarian stimulation on endo-metrial function. Semin Reprod Med. 2007;25(6):454–460.
Bourgain, C, Devroey, P The endometrium in stimulated cycles for Ivf. Hum Reprod Update. 2003;9(6):515–522.
Rizzo, G, Aiello, E, Pietrolucci, ME, Arduini, D Placental volume and uterine artery doppler evaluation at 11 + 0 to 13 + 6 weeks of gestation in pregnancies conceived with in vitro fertilization: comparison between autologous and donor oocyte recipients. Ultrasound Obstet Gynecol. 2016;47(6):726–731.
Kansal Kalra, S, Ratcliffe, SJ, Milman, L, Gracia, CR, Coutifaris, C, Barnhart, KT Perinatal morbidity after in vitro fertilization is lower with frozen embryo transfer. Fertil Steril. 2011;95(2): 548–553.
Plasencia, W, Maiz, N, Bonino, S, Kaihura, C, Nicolaides, K Uterine artery Doppler at 11 + 0 to 13 + 6 weeks in the prediction of pre-eclampsia. Ultrasound Obstet Gynecol. 2007;30(5):742–749.
Schuchter, K, Metzenbauer, M, Hafner, E, Philipp, K Uterine artery Doppler and placental volume in the first trimester in the predic-tion of pregnancy complications. Ultrasound Obstet Gynecol. 2001;18(6):590–592.
Odibo, AO, Goetzinger, KR, Huster, KM, Christiansen, JK, Odibo, L, Tuuli, MG Placental volume and vascular flow assessed by 3D power Doppler and adverse pregnancy outcomes. Placenta. 2011; 32(3):230–234.
Brown, MA, Lindheimer, MD, de Swiet, M, Van Assche, A, Mout-quin, JM The classification and diagnosis of the hypertensive disorders of pregnancy: statement from the international society for the study of hypertension in pregnancy (ISSHP). Hypertens Pregnancy. 2001;20(1):IX–XIV.
Bertino, E, Spada, E, Occhi, L, et al. Neonatal anthropometric charts: the Italian neonatal study compared with other European studies. J Pediatr Gastroenterol Nutr. 2010; 51(3):353–361.
Rizzo, G, Capponi, A, Cavicchioni, O, Vendola, M, Arduini, D First trimester uterine Doppler and three-dimensional ultrasound pla-cental volume calculation in predicting pre-eclampsia. Eur J Obstet Gynecol Reprod Biol. 2008;138(2):147–151.
Wegrzyn, P, Faro, C, Falcon, O, Peralta CFA, Nicolaides, KH Pla-cental volume measured by three-dimensional ultrasound at 11 to 13 + 6 weeks of gestation: relation to chromosomal defects. Ultrasound Obstet Gynecol. 2005;26(1):28–32.
Rizzo, G, Capponi, A, Pietrolucci, ME, Arduini, D Effects of maternal cigarette smoking on placental volume and vascularization measured by 3-dimensional power Doppler ultrasonography at 11+0 to 13+6 weeks of gestation. Am J Obstet Gynecol. 2009;200(4):415. e1-e5.
Brosens, I, Pijnenborg, R, Vercruysse, L, Romero, R The ‘Great Obstetrical Syndromes’ are associated with disorders of deep pla-centation. Am J Obstet Gynecol. 2011;204(3):193–201.
Khalil, A, Cowans, NJ, Spencer, K, Goichman, S, Meiri, H, Harrington, K First-trimester markers for the prediction of preeclampsia in women with a-priori high risk. Ultrasound Obstet Gynecol. 2010;35(6):671–679.
Velauthar, L, Plana, MN, Kalidindi, M, et al. First-trimester uterine artery Doppler and adverse pregnancy outcome: a meta-analysis involving 55 974 women. Ultrasound Obstet Gynecol. 2014; 43(5):500–507.
Prefumo, F, Fratelli, N, Soares, SC, Thilaganathan, B Uterine artery Doppler velocimetry at 11–14 weeks in singleton pregnancies conceived by assisted reproductive technology. Ultrasound Obstet Gynecol. 2007;29(2):141–145.
Carbone, IF, Cruz, JJ, Sarquis, R, Akolekar, R, Nicolaides, KH Assisted conception and placental perfusion assessed by uterine artery Doppler at 11–13 weeks’ gestation. Hum Reprod. 2011; 26(7):1659–1664.
Haapsamo, M, Martikainen, H, Tinkanen, H, Heinonen, S, Nuojua-Huttunen, S, Räsänen, J Low-dose aspirin therapy and hypertensive pregnancy complications in unselected IVF and ICSI patients: a randomized, placebo-controlled, double-blind study. Hum Reprod. 2010;25(12):2972–2977.
Hafner, E, Metzenbauer, M, Höfinger, D, et al. Comparison between three-dimensional placental volume at 12 weeks and uterine artery impedance/notching at 22 weeks in screening for pregnancy-induced hypertension, pre-eclampsia and fetal growth restriction in a low-risk population. Ultrasound Obstet Gynecol. 2006;27:652–657.
Choux, C, Carmignac, V, Bruno, C, Sagot, P, Vaiman, D, Fauque, P The placenta: phenotypic and epigenetic modifications induced by Assisted Reproductive Technologies throughout pregnancy. Clin Epigenetics. 2015;7(1):87.
Hafner, E, Metzenbauer, M, Höfinger, D, et al. Placental growth from the first to the second trimester of pregnancy in SGA-foetuses and pre-eclamptic pregnancies compared to normal fetuses. Placenta. 2003:24(4):336–342.
Wright, D, Akolekar, R, Syngelaki, A, Poon, LC, Nicolaides, KH A competing risks model in early screening for preeclampsia. Fetal Diagn Ther. 2012;32(3):171–178.
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Rizzo, G., Aiello, E., Pietrolucci, M.E. et al. Are There Differences in Placental Volume and Uterine Artery Doppler in Pregnancies Resulting From the Transfer of Fresh Versus Frozen-Thawed Embryos Through In Vitro Fertilization. Reprod. Sci. 23, 1381–1386 (2016). https://doi.org/10.1177/1933719116641765
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DOI: https://doi.org/10.1177/1933719116641765