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rhTNFR: Fc Suppresses the Development of Endometriosis in a Mouse Model by Downregulating Cell Proliferation and Invasiveness

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Abstract

Tumor necrosis factor α (TNF-α), a proinflammatory cytokine, may play an important role in the pathogenesis of endometriosis; therefore, TNF-α inhibitors potentially have an effect on endometriosis. To investigate the effect of anti–TNF-α treatment on endometriosis, 2 TNF-α inhibitors: recombinant human TNF receptor: Fc fusion protein (rhTNFR: Fc) and TNF-α monoclonal antibody (TNF-α mAb) were used to treat human eutopic endometrial stromal cells (hESCs), and the effects on cell survival, cell cycle, and invasiveness were compared. It was found that rhTNFR: Fc suppressed the TNF-α–induced hESC survival and invasiveness but not TNF-α mAb. Recombinant human TNF receptor: Fc fusion protein decreased the S phase of hESC compared with the TNF-α–treated group. Then, we used a surgically induced mouse model of endometriosis to study the effect of rhTNFR: Fc treatment in vivo. The fluorescence intensity and the size of implanted endometriotic lesions in the mouse model were decreased by rhTNFR: Fc. In conclusion, rhTNFR: Fc suppresses hESC survival and invasiveness and decreases the fluorescence intensity and implant size in the mouse model of endometriosis.

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Authors and Affiliations

  1. The State Key Laboratory of Reproductive Medicine, Clinical Center of Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, 300 Guangzhou Road, 210029, Nanjing, China

    Ying Liu MD, Zhen Hou MD, Yundong Mao MD, Yugui Cui MD & Jiayin Liu MD

  2. Hangzhou Maternal and Child Health Hospital, Hangzhou, China

    Liang Sun PhD

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  1. Ying Liu MD
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  2. Liang Sun PhD
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  3. Zhen Hou MD
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  6. Jiayin Liu MD
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Correspondence to Ying Liu MD.

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Liu, Y., Sun, L., Hou, Z. et al. rhTNFR: Fc Suppresses the Development of Endometriosis in a Mouse Model by Downregulating Cell Proliferation and Invasiveness. Reprod. Sci. 23, 847–857 (2016). https://doi.org/10.1177/1933719115620495

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