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Serum MicroRNAs as Diagnostic Biomarkers for Macrosomia

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Abstract

Background

Macrosomia is defined as an infant’s birth weight of more than 4000 g. Although microRNAs (miRNAs) have been implicated in the pathogenesis of various diseases, the associations between serum miRNAs and macrosomia have been rarely reported.

Methodology

We used the Taqman Low Density Array followed by quantitative reverse transcriptase polymerase chain reaction assays to screen for miRNAs associated with macrosomia using serum samples collected 1 week before delivery.

Results

Profiling results showed that 1 miRNA was significantly upregulated and 10 miRNAs were significantly downregulated in serum samples of macrosomia (ΔΔCt > 3-fold). The expression levels of miR-21 were significantly decreased in macrosomia as compared to the controls in the third trimester. Receiver operating characteristic (ROC) curve analyses showed that the area under the ROC curve for miR-21 was 67.7% (sensitivity = 66.7% and specificity = 70.0%).

Conclusions

miR-21 in maternal serum is differentially expressed between macrosomia and controls, and miR-21 could be used as a candidate biomarker to predict macrosomia.

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Authors and Affiliations

  1. Perinatology Unit, Changzhou Maternity and Child Health Care Hospital affiliated to Nanjing Medical University, Changzhou, Jiangsu, P.R. China

    Hua Jiang, Lingmin Hu, Tingting Miao & Ming Zhang

  2. Department of Epidemiology and Biostatistics, State Key Lab of Reproductive Medicine, School of Public Health, Nanjing Medical University, Nanjing, China

    Yang Wen & Jing Dong

Authors
  1. Hua Jiang
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  2. Yang Wen
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  3. Lingmin Hu
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  4. Tingting Miao
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  5. Ming Zhang
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  6. Jing Dong
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Correspondence to Hua Jiang.

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Cite this article

Jiang, H., Wen, Y., Hu, L. et al. Serum MicroRNAs as Diagnostic Biomarkers for Macrosomia. Reprod. Sci. 22, 664–671 (2015). https://doi.org/10.1177/1933719114561557

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