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Establishment of a New Ovarian Cancer Cell Line CA5171

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Abstract

A new cell line, CA5171, derived from a chemotherapy-naive, high-grade undifferentiated ovarian carcinoma was established and characterized. The CA5171 cells presented with cobblestone morphology and a doubling time of 24 hours. Gene mutation analysis showed that the cells belonged to the type II ovarian cancer pathway with mutations of PIK3CA, PTEN, and TP53. Single-nucleotide polymorphism array analysis showed no homozygous gene deletion; however, several loci of gene copy number gains were noted in chromosome 1, 2, 5, 9, 10, 12, 15, 16, 20, and X. The in vitro and in vivo experiments showed that the cells were sensitive to paclitaxel and doxorubicin, but resistant to cisplatin. The cells also presented epithelial-mesenchymal transition properties that may have been related to their invasion and migration potential. The CA5171 cells show the potential as a new cell line for studies on epithelial ovarian carcinoma.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, College of Medicine, National Taiwan University, No. 8, Chung-Shan South Road, Taipei, Taiwan

    Ying-Cheng Chiang MD, Wen-Fang Cheng MD, PhD, Ming-Cheng Chang PhD, Hsiu-Ping Lin BS, Chang-Yao Hsieh MD, MSPH & Chi-An Chen MD

  2. Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan

    Ying-Cheng Chiang MD & Wen-Fang Cheng MD, PhD

  3. Graduate Institute of Oncology, College of Medicine, National Taiwan University, Taipei, Taiwan

    Wen-Fang Cheng MD, PhD

  4. Department of Public Health, Graduate Institute of Epidemiology and Preventive Medicine, National Taiwan University, Taipei, Taiwan

    Tzu-Pin Lu PhD

  5. Department of Pathology, National Taiwan University Hospital, Taipei, Taiwan

    Kuan-Ting Kuo MD

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  1. Ying-Cheng Chiang MD
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  2. Wen-Fang Cheng MD, PhD
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  3. Ming-Cheng Chang PhD
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  5. Kuan-Ting Kuo MD
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  6. Hsiu-Ping Lin BS
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  7. Chang-Yao Hsieh MD, MSPH
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  8. Chi-An Chen MD
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Correspondence to Chi-An Chen MD.

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Chiang, YC., Cheng, WF., Chang, MC. et al. Establishment of a New Ovarian Cancer Cell Line CA5171. Reprod. Sci. 22, 725–734 (2015). https://doi.org/10.1177/1933719114557893

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  • DOI: https://doi.org/10.1177/1933719114557893

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