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Expression of Collapsin Response Mediator Protein 1 in Placenta of Normal Gestation and Link to Early-Onset Preeclampsia

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Abstract

A human isoform of Collapsin Response Mediator Protein (CRMP) family proteins, CRMP-1, has been identified as a novel invasion suppressor. The aim of this study was to determine CRMP-1 expression pattern in placentas during normal pregnancy and elucidate the clinical significance of CRMP-1 expression in the placentas of women with early-onset preeclamptic pregnancies. We recruited 66 normal healthy pregnant Chinese women and 60 Chinese patients with preeclampsia [early-onset prereclampsia(ePE), n = 30 and late-onset preeclampsia(lPE) n = 30]. Gestational age-matched normal healthy pregnant women were used as controls of early-onset and late-onset preeclampsia, which were 23–33 + 6weeks, n = 18 and control B: 34–40 weeks, n = 20). Quantitative RT-PCR, Western blot analysis and immunohistochemistry were used to analyze the expressions of CRMP-1 in placentas. Expression of CRMP-1 was detected in syncytio- and cytotrophoblasts of all groups using immunohistochemistry. CRMP-1 was most abundantly expressed in syncytiotrophoblasts, moderately in cytotrophoblasts and the intermediate trophoblasts especially in the first trimester. The placental expression of CRMP-1 is particularly striking in the first trimester and decreases throughout pregnancy. There is a significant increase in CRMP-1 expression in the placenta of ePE but not of lPE, as compared to gestational-matched controls. The aberrant upregulation of CRMP-1 expression may link to the mechanism of developing ePE.

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References

  1. Gou WL. Hypertensive disorder in pregnancy). In: Cao ZY, ed. Chinese Obstetrics and Gynecology, 2nd ed. Beijing: People’s Medical Publishing House Co., LTD; 2004:383–342.

    Google Scholar 

  2. Walker JJ. Pre-eclampsia. Lancet. 2000;356(9237):1260–1265.

    Article  CAS  Google Scholar 

  3. Meekins JW, Pijnenborg R, Hanssens M, McFadyen IR, van Asshe A. A study of placental bed spiral arteries and Trophoblast invasion in normal and severe preeclamptic pregnancies. Br J Obstet Gynaecol. 1994;101(8):669–674.

    Article  CAS  Google Scholar 

  4. Von Dadelszen P, Magee LA, Roberts JM. Subclassification of preeclampsia. Hypertens Pregnancy. 2003;22(2):143–148.

    Article  Google Scholar 

  5. Eastabrook G, Brown M, Sargent I. The origins and end-organ consequence of pre-eclampsia. Best Pract Res Clin Obstet Gynecol. 2011;25(4):435–447.

    Article  Google Scholar 

  6. Huppertz B. Placental origins of preeclampsia: challenging the current hypothesis. Hypertension. 2008;51(4):970–975.

    Article  CAS  Google Scholar 

  7. Raymond D, Peterson E. A critical review of early-onset and late-onset preeclampsia. Obstet Gynecol Surv. 2011;66(8):497–506.

    Article  Google Scholar 

  8. Qiao C, Wang C, Zhao J, Liu C, Shang T. Elevated expression of KiSS-1 in placenta of Chinese women with early-onset preeclampsia. PLoS One. 2012;7(11):e48937. doi:10.1371/journal.pone.0048937.

    Article  CAS  Google Scholar 

  9. Oudejans CB, van Dijk M, Oosterkamp M, Lachmeijer A, Blankenstein MA. Genetics of preeclampsia: paradigm shifts. Hum Genet. 2007;120(5):607–612.

    Article  Google Scholar 

  10. Crispi F, Llurba E, Domínguez C, Martín-Gallán P, Cabero L, Gratacós E. Predictive value of angiogenic factors and uterine artery Doppler for early-versus late-onset preeclampsia and intrauterine growth restriction. Ultrasound Obstet Gynecol. 2008;31(3):303–309.

    Article  CAS  Google Scholar 

  11. Rosslenbroich V, Dai L, Baader SL, Noegel AA, Gieselmann V, Kappler J. Collapsin response mediator protein-4 regulates F-actin bundling. Exp Cell Res. 2005;310(2):434–444.

    Article  CAS  Google Scholar 

  12. Quinn CC, Gray GE, Hockfield S. A family of proteins implicated in axon guidance and outgrowth. J Neurobiol. 1999;41(1):158–164.

    Article  CAS  Google Scholar 

  13. Shih JY, Lee YC, Yang SC, Hong TM, Huang CY, Yang PC. Collapsin response mediator protein-1: a novel invasion-suppressor gene. Clin Exp Metastasis. 2003;20(1):69–76.

    Article  CAS  Google Scholar 

  14. Goshima Y, Nakamura F, Strittmatter P, Strittmatter SM. Collapsin-induced growth cone collapse mediated by an intracellular protein related to UNC-33. Nature. 1995;376(6540):509–514.

    Article  CAS  Google Scholar 

  15. Wang LH, Strittmatter SM. Brain CRMP forms heterotetramers similar to liver dihydropyrimidinase. J Neurochem. 1997;69(6):2261–2269.

    Article  CAS  Google Scholar 

  16. Fukada M, Watakabe I, Yuasa-Kawada J, et al. Molecular characterization of CRMP5, a novelmember of the collapsin response mediator protein family. J Biol Chem. 2000;275(48):37957–37965.

    Article  CAS  Google Scholar 

  17. Arimura N, Inagaki N, Chihara K, et al. Phosphorylation of collapsin response mediator protein-2 by Rho-kinase Evidence for two separate signaling pathways for growth cone collapse. J Biol Chem. 2000;275(31):23973–23980.

    Article  CAS  Google Scholar 

  18. Shih JY, Yang SC, Hong TM, et al. Collapsin response mediator protein-1 and the invasion and metastasis of cancer cells. J Natl Cancer Inst. 2001;93(18):1392–1400.

    Article  CAS  Google Scholar 

  19. Chen JJ, Peck K, Hong TM, et al. Global analysis of gene expression in invasion by a lung cancer model. Cancer Res. 2001; 61(13):5223–5230.

    CAS  PubMed  Google Scholar 

  20. Gauster M, Moser G, Orendi K, Huppertz B. Factors involved in regulating trophoblast fusion: potential role in the development of preeclampsia. Placenta. 2009;30(suppl A):S49–S54.

    Article  Google Scholar 

  21. Matsuo T, Stauffer JK, Walker RL, Meltzer P, Thiele CJ. Structure and promoter analysis of the human unc-33-like phosphoprotein gene: E-box required for maximal expression in neuroblastoma and myoblasts. J Biol Chem. 2000;275(32):16560–16568.

    Article  CAS  Google Scholar 

  22. Inagaki H, Kato Y, Hamajima N, Nonaka M, Sasaki M, Eimoto T. Differential expression of dihydropyrimidinase-related protein genes in developing and adult enteric nervous system. Histochem Cell Biol. 2000;113(1):37–41.

    Article  CAS  Google Scholar 

  23. Raper JA. Semaphorins and their receptors in vertebrates and invertebrates. Curr Opin Neurobiol. 2000;10(1):88–94.

    Article  CAS  Google Scholar 

  24. Carty DM, Delles C, Dominiczak AF. Novel biomarkers for predicting preeclampsia. Trends Cardiovasc Med. 2008;18(5):186–194.

    Article  CAS  Google Scholar 

  25. Wikström AK, Larsson A, Eriksson UJ, Nash P, Olovsson M. Early postpartum changes in circulating pro- and antiangiogenic factors in early-onset and late-onset preeclampsia. Acta Obstetet Gynecol. 2008;87(2):146–153.

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Shengjing Hospital, China Medical University, No.36, Sanhao street, 110004, Shenyang, China

    Chong Qiao MD, PhD, Feng Jin MD, Dongying Zheng & Caixia Liu

  2. Department of Hepatobiliary Surgery, General Hospital of Shenyang Military Region, Shenyang, China

    Chunhui Wang MD, PhD

Authors
  1. Chong Qiao MD, PhD
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  2. Chunhui Wang MD, PhD
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  3. Feng Jin MD
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  4. Dongying Zheng
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  5. Caixia Liu
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Correspondence to Chong Qiao MD, PhD.

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Qiao, C., Wang, C., Jin, F. et al. Expression of Collapsin Response Mediator Protein 1 in Placenta of Normal Gestation and Link to Early-Onset Preeclampsia. Reprod. Sci. 22, 495–501 (2015). https://doi.org/10.1177/1933719114549847

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