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FAS and FAS-L Genotype and Expression in Patients With Recurrent Pregnancy Loss

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Abstract

We assessed FAS and FAS-L gene polymorphisms and messenger RNA (mRNA) levels in patients with recurrent pregnancy loss (RPL). This case–control study compared 129 women with RPL with 235 healthy multiparous women (control group). Genomic DNA and total mRNA were extracted from whole blood, and polymorphisms genotyping was performed by polymerase chain reaction (PCR). Messenger RNA expression levels were analyzed by real-time PCR. Data were analyzed by chi-square and Fisher exact tests; P < .05 was considered significant. There were no significant differences in the FAS (670 A/G) genotype or allelic frequencies between the RPL and control groups. We found significant differences in the FAS-L (844 C/T) genotype and allelic frequencies between women with RPL and controls. Patients with RPL had significantly higher FAS-L expression. Our data suggest that FAS-L gene polymorphism is associated with increased susceptibility to RPL. Moreover, women with RPL seem to abnormally express FAS-FAS-L molecules.

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Authors and Affiliations

  1. Obstetrics Department, Universidade Federal de São Paulo, Rua Diogo de Faria 477 ap31, CEP04037-001, São Paulo, SP, Brazil

    Priscilla Chamelete Andrade Banzato MD, Silvia Daher MD, PhD, Évelyn Traina MD, PhD, Maria Regina Torloni MD, PhD, Bárbara Yasmin Gueuvoghlanian-Silva MSc, Renata Fiorini Puccini MD, Karen Priscilla Tezotto Pendeloski MSc & Rosiane Mattar MD, PhD

Authors
  1. Priscilla Chamelete Andrade Banzato MD
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  2. Silvia Daher MD, PhD
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  3. Évelyn Traina MD, PhD
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  4. Maria Regina Torloni MD, PhD
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  5. Bárbara Yasmin Gueuvoghlanian-Silva MSc
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  6. Renata Fiorini Puccini MD
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  7. Karen Priscilla Tezotto Pendeloski MSc
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  8. Rosiane Mattar MD, PhD
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Correspondence to Rosiane Mattar MD, PhD.

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Banzato, P.C.A., Daher, S., Traina, É. et al. FAS and FAS-L Genotype and Expression in Patients With Recurrent Pregnancy Loss. Reprod. Sci. 20, 1111–1115 (2013). https://doi.org/10.1177/1933719113477488

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  • DOI: https://doi.org/10.1177/1933719113477488

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