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Role of Mitochondria in Ciprofloxacin-Induced Apoptosis in Murine Sperm Cells

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Abstract

Rationale

Ciprofloxacin (CPFX) has been reported to inhibit cell growth and induce apoptosis in certain eukaryotic cells. The role of the mitochondrial pathway in CPFX-induced apoptosis in cultured murine sperm cells was investigated.

Methods and Results

Sperm cells (5×103 cells/well) from 8-week-old NMRI male mice were cultured in 150 μL of HAM’s F10 with 25 mmol/L (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) HEPES and 10% human serum albumin and were incubated with 50, 100, 200, 400, and 800 µg/mL CPFX for 24 and 36 hours. Cell cytotoxicity, mitochondrial membrane potential (ΔΨM), and concentrations of caspase 3 and caspase 9 were assessed in CPFX-treated cultured murine sperm cells by MTT (3-(4,5-dimethylthiazol-2-Yl)-2,5-diphenyltetrazolium bromide) , JC-1 (5, 5á, 6, 6á-tetrachloro-1, 1á, 3, 3á-tetraethylbenzimidazol-carbocyanine iodide)aggregation, and caspase 3 and caspase 9 assays, respectively. Increasing doses of CPFX showed significant cytotoxicity (EC50 = 146.73 μg/mL). Significant loss of ΔΨm was observed in sperm cells treated with ≥50 μg/mL CPFX for 36 hours. Significant increases in caspase 9 and caspase 3 concentrations were also found in cells treated with ≥50 μg/mL CPFX for 24 and 36 hours, respectively (P < .001).

Conclusions

Effects of clinically reachable doses of CPFX on cultured murine sperm cells were investigated and revealed that it may cause sperm cell toxicity by induction of apoptosis through the mitochondrial pathway in the clinically reachable concentrations.

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Authors and Affiliations

  1. Department of Basic Sciences, Mahabad Branch, Islamic Azad University, Mahabad, Iran

    Fatemeh Zobeiri DVM, PhD

  2. Department of Clinical Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Siamak Salami PhD

  3. Department of Basic Sciences, Histology and Embryology Section, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

    Rajabali Sadrkhanlou DVM, PhD

  4. Department of Histology and Embryology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran

    Tahmineh Peirouvi PhD

Authors
  1. Fatemeh Zobeiri DVM, PhD
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  2. Siamak Salami PhD
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  3. Rajabali Sadrkhanlou DVM, PhD
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  4. Tahmineh Peirouvi PhD
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Correspondence to Siamak Salami PhD.

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Zobeiri, F., Salami, S., Sadrkhanlou, R. et al. Role of Mitochondria in Ciprofloxacin-Induced Apoptosis in Murine Sperm Cells. Reprod. Sci. 20, 1090–1095 (2013). https://doi.org/10.1177/1933719113477482

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