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Granulocyte—Macrophage Colony-Stimulating Factor Enhances the Humoral Immune Responses of Mouse Zona Pellucida 3 Vaccine Strategy Based on DNA and Protein Coadministration in BALB/c Mice

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Abstract

We recently demonstrated that co-administration of mouse zona pellucida 3 (mZP3) DNA and protein vaccine enhanced the contraception of mice by increasing humoral immune responses. In this study, we try to use granulocyte—macrophage colony-stimulating factor (GM-CSF) to further improve the humoral immune responses induced by mZP3 DNA and protein co-administration. BALB/c mice were intranasally pre-injected with GM-CSF 4 days before co-administration. Compared to DNA and protein coadministration without GM-CSF, the combination of GM-CSF and coadministration significantly enhances humoral immune responses, especially the level of secretory immunoglobulin A (sIgA) in vaginal washes. The enhanced antibody responses are correlated with the upregulated level of interleukin 4 (IL-4) and enhanced maturation of dendritic cells (DCs). Thus, GM-CSF is a potential candidate adjuvant to be used for the development of a safe and effective contraceptive vaccine.

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Authors and Affiliations

  1. Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, 14 Shengli Road, 830046, Urumqi, China

    Zibirnisa Kadir MD, Xiaolin Ma MS & Fuchun Zhang PhD

  2. Human Retrovirus Section, Vaccine Branch, Center for Cancer Research, Frederick National Laboratory for Cancer Research, 1050 Boyles Street, Building 535, Room 226, 21702, Frederick, MD, USA

    Jinyao Li PhD

Authors
  1. Zibirnisa Kadir MD
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  2. Xiaolin Ma MS
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  3. Jinyao Li PhD
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  4. Fuchun Zhang PhD
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Correspondence to Jinyao Li PhD or Fuchun Zhang PhD.

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Kadir, Z., Ma, X., Li, J. et al. Granulocyte—Macrophage Colony-Stimulating Factor Enhances the Humoral Immune Responses of Mouse Zona Pellucida 3 Vaccine Strategy Based on DNA and Protein Coadministration in BALB/c Mice. Reprod. Sci. 20, 400–407 (2013). https://doi.org/10.1177/1933719112459236

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  • DOI: https://doi.org/10.1177/1933719112459236

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