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Association of miR-146aC>G, miR-196a2T>C, and miR-499A>G Polymorphisms With Risk of Premature Ovarian Failure in Korean Women

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Abstract

We investigated whether microRNA (miRNA) polymorphisms (miR-146aC>G, miR-196a2T>C, and miR-499A>G) confer risk of premature ovarian failure (POF) in Korean women. DNA samples from 136 patients with POF and 234 controls were genotyped for the 3 miRNA single-nucleotide polymorphisms by polymerase chain reaction-restriction fragment length polymorphism. The miR-146aCG/miR-196a2TC combined genotype was less frequent in patients than in controls (P < .05), conferring less susceptibility. Using haplotype-based multifactor dimensionality reduction analysis, the C-C-A and G-T-A inferred haplotypes (miR-146a/miR-196a2/miR-499) were less frequent in patients, suggesting protective effects (P < .05 for each), whereas the C-T-A and G-C-A haplotypes were more frequent in patients (P < .05 for each). The C-T and G-C haplotypes (miR-146a/miR-196a2) were more frequent in patients, whereas the C-C and G-T haplotypes were less frequent in patients (P < .05 for each). However, none of the 3 miRNA polymorphisms alone was associated with POF risk. Our findings suggest that putative gene-gene interaction between miR-146 and miR-196a2 may be involved in POF development.

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Authors and Affiliations

  1. Department of Biomedical Science, College of Life Science, CHA University, Seongnam, South Korea

    HyungChul Rah DVM, PhD, Young Joo Jeon MS, Sung Han Shim PhD & Nam Keun Kim PhD

  2. Institute for Clinical Research, CHA Bundang Medical Center, CHA University, 351 Yatap-dong, Bundang-gu, 463-712, Seongnam, South Korea

    HyungChul Rah DVM, PhD, Young Joo Jeon MS & Nam Keun Kim PhD

  3. Fertility Center of CHA Gangnam Medical Center, CHA University, Seoul, South Korea

    Sung Han Shim PhD

  4. Department of Obstetrics and Gynecology, CHA Bundang Medical Center, CHA University, Seongnam, South Korea

    Sun Hee Cha MD, PhD, Dong Hee Choi MD, PhD, Hwang Kwon MD, PhD, Ji Hyang Kim MD & Ji Eun Shin MD

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  1. HyungChul Rah DVM, PhD
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Rah, H., Jeon, Y.J., Shim, S.H. et al. Association of miR-146aC>G, miR-196a2T>C, and miR-499A>G Polymorphisms With Risk of Premature Ovarian Failure in Korean Women. Reprod. Sci. 20, 60–68 (2013). https://doi.org/10.1177/1933719112450341

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