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Epab and Pabpc1 Are Differentially Expressed During Male Germ Cell Development

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Abstract

Modification of poly(A) tail length constitutes the main posttranscriptional mechanism by which gene expression is regulated during spermatogenesis. Embryonic poly(A)-binding protein (EPAB) and somatic cytoplasmic poly(A)-binding protein (PABPC1) are the 2 key proteins implicated in this pathway. In this study we characterized the temporal and spatial expression of Epab and Pabpc1 in immature (D6-D32) and mature (D88) mouse testis and in isolated spermatogenic cells. Both Epab and Pabpc1 expression increased during early postnatal life and reached their peak at D32 testis. This was due to an increase in both spermatogonia (SG) and spermatocytes. In the mature testis, the highest levels of Epab were detected in SG, followed by round spermatids (RSs), while the most prominent Pabpc1 expression was detected in spermatocytes and RSs. Our findings suggest that PABPC1 may play a role in translational regulation of gene expression by cytoplasmic polyadenylation, which occurs in spermatocytes, while both EPAB and PABPC1 may help stabilize stored polyadenylated messenger RNAs in RSs.

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Authors and Affiliations

  1. Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, 310 Cedar Street, LSOG 304D, New Haven, CT, 06520-8063, USA

    Saffet Ozturk MSc, Ozlem Guzeloglu-Kayisli PhD, Orkan Ilbay BSc, Maria D. Lalioti PhD & Emre Seli MD

  2. Department of Histology and Embryology, Akdeniz University School of Medicine, Campus, Antalya, Turkey

    Saffet Ozturk MSc, Necdet Demir PhD & Berna Sozen BSc

Authors
  1. Saffet Ozturk MSc
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  2. Ozlem Guzeloglu-Kayisli PhD
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  3. Necdet Demir PhD
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  4. Berna Sozen BSc
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  5. Orkan Ilbay BSc
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  6. Maria D. Lalioti PhD
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  7. Emre Seli MD
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Correspondence to Emre Seli MD.

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Ozturk, S., Guzeloglu-Kayisli, O., Demir, N. et al. Epab and Pabpc1 Are Differentially Expressed During Male Germ Cell Development. Reprod. Sci. 19, 911–922 (2012). https://doi.org/10.1177/1933719112446086

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