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Resveratrol Prevents Impairment in Activation of Retinoic Acid Receptors and MAP Kinases in the Embryos of a Rodent Model of Diabetic Embryopathy

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Abstract

Diabetes induces impairments in gene expression during embryonic development that leads to premature and improper tissue specialization. Retinoic acid receptors (RARs and retinoid X receptor [RXRs]) and mitogen-activated protein kinases (MAPKs) play crucial roles during embryonic development, and their suppression or activation has been shown as a determinant of the fate of embryonic organogenesis. We studied the activation of RARs and MAPKs in embryonic day 12 (E12) in embryos of rats under normal, diabetic, and diabetic treated with resveratrol ([RSV]; 100 mg/kg body weight) conditions. We found downregulation of RARs and RXRs expressions as well as their DNA-binding activities in the embryos exhibiting developmental delays due to diabetes. Furthermore, the phosphorylation of extracellular signal-regulated kinase (ERK) 1/2 was decreased and phosphorylation of c-Jun N-terminal kinase (JNK) 1/2 and p38 was increased. Interestingly, embryos of diabetic rats treated with RSV showed normalized patterns of RARs, RXRs, neuronal markers, and ERK, JNK and p38 phosphorylation.

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Authors and Affiliations

  1. Department of Pathology, Microbiology and Immunology, School of Medicine, University of South Carolina, 6311 Garners Ferry Road, Columbia, SC, 29209, USA

    Chandra K. Singh PhD, Ambrish Kumar PhD & Ugra S. Singh PhD

  2. Department of Cell Biology and Anatomy, School of Medicine, University of South Carolina, Columbia, SC, USA

    Holly A. LaVoie PhD & Donald J. DiPette MD

  3. Department of Dermatology, University of Wisconsin, 1300 University, Avenue, MSC 424, Madison, WI, 53706, USA

    Chandra K. Singh PhD

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  1. Chandra K. Singh PhD
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  4. Donald J. DiPette MD
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Correspondence to Ugra S. Singh PhD.

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Singh, C.K., Kumar, A., LaVoie, H.A. et al. Resveratrol Prevents Impairment in Activation of Retinoic Acid Receptors and MAP Kinases in the Embryos of a Rodent Model of Diabetic Embryopathy. Reprod. Sci. 19, 949–961 (2012). https://doi.org/10.1177/1933719112438972

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