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Expression of Keratinocyte Growth Factor in Goat Ovaries and Its Effects on Preantral Follicles Within Cultured Ovarian Cortex

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Abstract

The aims of this study were to evaluate the expression of keratinocyte growth factor (KGF) in goat ovaries and to study its effects on preantral follicle survival and development. The ovaries were used for immunohistochemistry or for in vitro culture for 1 or 7 days with KGF (0, 1, 10, 50, 100, 150, or 200 ng/mL). Noncultured (fresh control) and cultured ovarian slices were processed for histological analysis and transmission electron microscopy (TEM). The results showed that after 7 days of in vitro culture, all treatments had a significant reduction in the percentage of normal follicles compared with the fresh control. After 7 days of culture, the highest KGF concentrations (150 and 200 ng/mL) induced a significant reduction in the percentage of normal follicles compared with the tissues cultured in the absence (α-MEM+ alone) or presence of 1, 10, and 50 ng/mL KGF. Transmission electron microscopy confirmed follicular integrity after 7 days of culture in 1 ng/mL KGF. In addition, compared with the fresh control, the percentage of growing follicles was significantly increased in all treatments after 1 or 7 days of culture. Immunohistochemical analyses showed the expression of KGF in oocytes and granulosa cells in all follicle developmental stages as well as in thecal and stromal cells. In conclusion, this study demonstrated that, at the lowest concentration (1 ng/mL), KGF maintained the ultrastructure of goat preantral follicles cultured in vitro for up to 7 days. Furthermore, the KGF protein was widely distributed in goat ovaries, especially in ovarian follicles.

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Authors and Affiliations

  1. Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary, State University of Ceará, Fortaleza, CE, Brazil

    L. R. Faustino MS, R. Rossetto MS, I. M. T. Lima MS, C. M. G. Silva MS, M. V. A. Saraiva MS, L. F. Lima MS, C. C. Campello PhD, J. R. Figueiredo PhD & A. P. R. Rodrigues PhD

  2. Biotechnology Nucleus of Sobral (NUBIS), Federal University of Ceará, Sobral, CE, Brazil

    A. W. B. Silva MS

  3. Laboratory of Ultrastructure, CPqAM/FIOCRUZ, Federal University of Pernambuco, Recife-PE, Brazil

    M. A. M. Donato MS & C. A. Peixoto PhD

  4. Programa de Pós-Graduação em Ciências Veterinárias (PPGCV), Laboratório de Manipulação de Oócitos e Folículos Pré-Antrais (LAMOFOPA), Universidade Estadual do Ceará (UECE), Av. Paranjana, 1700, Campus do Itaperi, Fortaleza, CE, CEP: 60740-930, Brazil

    L. R. Faustino MS

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  1. L. R. Faustino MS
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  2. R. Rossetto MS
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  3. I. M. T. Lima MS
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  4. C. M. G. Silva MS
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  5. M. V. A. Saraiva MS
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  6. L. F. Lima MS
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  7. A. W. B. Silva MS
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  8. M. A. M. Donato MS
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  9. C. C. Campello PhD
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  10. C. A. Peixoto PhD
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  11. J. R. Figueiredo PhD
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  12. A. P. R. Rodrigues PhD
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Correspondence to L. R. Faustino MS.

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Faustino, L.R., Rossetto, R., Lima, I.M.T. et al. Expression of Keratinocyte Growth Factor in Goat Ovaries and Its Effects on Preantral Follicles Within Cultured Ovarian Cortex. Reprod. Sci. 18, 1222–1229 (2011). https://doi.org/10.1177/1933719111410709

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  • DOI: https://doi.org/10.1177/1933719111410709

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