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Remission of Endometriosis by Hyperbaric Oxygen Treatment in Rats

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Abstract

We designed this prospective, randomized controlled animal study to determine the effects of hyperbaric oxygen (HBO) on experimentally induced endometriosis in a rat model. Surgical induction of endometriosis was performed in 40, nonpregnant, female, Wistar-Albino rats at the Experimental Medicine Research Center of Istanbul University (DETAE). Four weeks later, the first and second laparotomies for volume measurement and peritoneal fluid (PF) collection were performed, and the rats were divided randomly into the study and control groups. The study group was exposed to HBO treatment for 6 weeks. Then, a third laparotomy was performed on all of the rats. The volume, histopathologic scores, Ki-67 labeling of the endometriotic implants, and the levels of tumor necrosis factor-α (TNF-α) in the PF were measured. The mean volume of the endometriotic implants in the study group was significantly lower than that of the control group at the end of the study (57.4 ± 12.5 vs 94.6 ± 17.2 mm3). The mean histopathological scores (1.60 ± 0.50 vs 2.42 ± 0.51), Ki-67 immunohistochemical scores (1.50 ± 0.51 vs 2.37 ± 0.49) of the endometriotic implants, and the TNF-α levels (5.33 ± 1.02 vs 8.16 ± 1.76 pg/mL) were significantly lower in the study group than in the control group. Hyperbaric oxygen treatment for 2 hours a day for 6 weeks resulted in significant remission of endometriosis in rats.

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References

  1. Benson RM, Minter LM, Osborne BA, Granowitz EV. Hyperbaric oxygen inhibits stimulus-induced proinflammatory cytokine synthesis by human blood-derived monocyte-macrophages. Clin Exp Immunol. 2003;134(1):57–62

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Fildissis G, Venetsanou K, Myrianthefs P, Karatzas S, Zidianakis V, Baltopoulos G. Whole blood pro-inflammatory cytokines and adhesion molecules post-lipopolysaccharides exposure in hyperbaric conditions. Eur Cytokine Netw. 2004;15(3):217–221

    CAS  PubMed  Google Scholar 

  3. Brenner I, Shepard RJ, Shek PN. Immune function in hyperbaric environments, diving, and decompression. Undersea Hyper Med. 1999;26(1):27–39

    CAS  Google Scholar 

  4. Murphy SA, Hyams JS, Fisher AB, Root RK. Effects of oxygen exposure on in vitro function of pulmonary alveolar macrophages. J Clin Invest. 1975;56(2):503–511

    Article  Google Scholar 

  5. Inamoto Y, Okuno F, Saito K, et al. Effect of hyperbaric oxygenation on macrophage function in mice. Biochem Biophys Res Comm. 1991;179(2):886–891

    Article  CAS  PubMed  Google Scholar 

  6. Lahat N, Bitterman H, Yaniv N, Kinarty A, Bitterman N. Exposure to hyperbaric oxygen induces tumour necrosis factor-alpha (TNF-a) secretion from rat macrophages. Clin Exp Immunol. 1995;102(3):655–659

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Yang Z, Nandi J, Wang J, et al. Hyperbaric oxygenation ameliorates indomethacin-induced enteropathy in rats by modulating TNF-alpha and IL-1beta production. Dig Dis Sci. 2006;51(8):1426–1433

    Article  CAS  PubMed  Google Scholar 

  8. Wu MY, Ho HN. The role of cytokines in endometriosis. Am J Reprod Immunol. 2003;49(3):285–296

    Article  PubMed  Google Scholar 

  9. Ethridge RT, Hashimoto K, Chung DH, Ehlers RA, Rajaraman S, Evers BM. Selective inhibition of NF-ΚB attenuates the severity of cerulein-induced acute pancreatitis. J Am Coll Surg. 2002;195(4):497–505

    Article  PubMed  Google Scholar 

  10. Gukovsky I, Gukovskaya AS, Blinman TA, Zaninovic V, Pandol SJ. Early NF kappa B activation is associated with hormone-induced pancreatitis. Am J Physiol. 1998;275(6 pt 1):1402–1414

    Google Scholar 

  11. Kim H, Seo JY, Roh KH, Lim JW, Kim KH. Suppression of NF-ΚB activation and cytokine production by N-acetylcysteine in pancreatic acinar cells. Free Rad Biol Med. 2000;29(7):674–683

    Article  CAS  PubMed  Google Scholar 

  12. Carswell EA, Old LJ, Kassel RL, Gren S, Fiore N, Williamson B. An endotoxin-induced serum factor that causes necrosis of tumors. Proc Natl Acad Sci USA. 1975;72(9):3666–3670

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Pennica D, Nedwin GE, Hayflick JS, Seeburg PH, Derynck R, Palladino MA. Human tumour necrosis factor: precursor structure, expression and homology to lymphotoxin. Nature. 1984;312(5996):724–729

    Article  CAS  PubMed  Google Scholar 

  14. Locksley RM, Killen N, Lenardo MJ. The TNF and TNF receptor superfamilies: integrating mammalian biology. Cell. 2001;104(4):487–501

    Article  CAS  PubMed  Google Scholar 

  15. Chen HL, Yang YP, Hu XL, Yelavarthi KK, Fishback JL, Hunt JC. Tumor necrosis factor alpha mRNA and protein are present in human placental and uterine cells at early and late stages of gestation. Am J Pathol. 1991;139(2):327–335

    CAS  PubMed  PubMed Central  Google Scholar 

  16. Jokhi PP, King A, Loke YW. Cytokine production and cytokine receptor expression by cells of the human first trimester placental-uterine interface. Cytokine. 1997;9(2):126–137

    Article  CAS  PubMed  Google Scholar 

  17. Bornstein SR, Rutkowski H, Vrezas I. Cytokines and steroidogenesis. Mol Cell Endocrinol. 2004;215(1–2):135–141

    Article  CAS  PubMed  Google Scholar 

  18. Wuttke W, Jarry H, Pitzel L, Knoke I, Spiess S. Luteotrophic and luteolytic actions of ovarian peptides. Hum Reprod. 1993;8(suppl 2):141–146

    Article  CAS  PubMed  Google Scholar 

  19. Terranova PF, Rice VM. Review: cytokine involvement in ovarian processes. Am J Reprod Immunol. 1997;37(1):50–63

    Article  CAS  PubMed  Google Scholar 

  20. Vernon MW, Wilson EA. Studies on the surgical induction of endometriosis in the rat. Fertil Steril. 1985;44(5):684–694

    Article  CAS  PubMed  Google Scholar 

  21. Keenan JA, Williams-Boyce PK, Massey PJ, Chen TT, Caudle MR. Bukovsky A regression of endometrial explants in a rat model of endometriosis treated with the immune modulators loxoribine and levamisole. Fertil Steril. 1999;72(1):135–141

    Article  CAS  PubMed  Google Scholar 

  22. Hunter S, Langemo DK, Anderson J, Hanson D, Thompson P. Hyperbaric oxygen therapy for chronic wounds. Adv Skin Wound Care. 2010;23(3):116–1169

    Article  PubMed  Google Scholar 

  23. Van den Blink B, van der Kleij AJ, Versteeg HH, Peppelenbosch MP. Immunomodulatory effect of oxygen and pressure. Comp Biochem Physiol Physiol. 2002;132(1):193–197

    Article  Google Scholar 

  24. Luongo C, Imperatore F, Cuzzocrea S, et al. Effects of hyperbaric oxygen exposure on a zymosan-induced shock model. Crit Care Med. 1998;26(12):1972–1976

    Article  CAS  PubMed  Google Scholar 

  25. Yamashita M, Yamashita M. Hyperbaric oxygen treatment attenuates cytokine induction after massive hemorrhage. Am J Physiol Endocrinol Metab. 2000;278(5):811–816

    Article  Google Scholar 

  26. Yang Z, Bosco G, Montante A, Ou X, Camporesi EM. Hyperbaric 02 reduces intestinal schemia-reperfusion-induced TNF-a production and lung neutrophil sequestration. Eur J Appl Physiol. 2001;85(1–2):96–103

    Article  CAS  PubMed  Google Scholar 

  27. Yamauchi N, Harada T, Taniguchi F, Yoshida S, Iwabe T, Terakawa N. Tumor necrosis factor-alpha induced the release of interleukin-6 from endometriotic stromal cells by the nuclear factor-kappaB and mitogen-activated protein kinase pathways. Fertil Steril. 2004;82(suppl 3):1023–1028

    Article  CAS  PubMed  Google Scholar 

  28. Yu X, Li YG, He XW, et al. Hyperbaric oxygen reduces inflammatory response in acute pancreatitis by inhibiting NF- kappaB activation. Eur Surg Res. 2009;42(2):130–135

    Article  CAS  PubMed  Google Scholar 

  29. Yu X, Zhang SD, Hang TQ. The change of pancreatic acinar cell function in murine acute necrotizing pancreatitis. Chin J Gen Surg. 2000;15(5):280–282

    Google Scholar 

  30. Wang F, He YL, Peng DX, Liu MB. Expressions of nuclear factor-kappaB and intercellular adhesion molecule-1 in endometriosis. Di Yi Jun Yi Da Xue Xue Bao. 2005;25(6):703–705

    CAS  PubMed  Google Scholar 

  31. Horie S, Harada T, Mitsunari M, Taniguchi F, Iwabe T, Terakawa N. Progesterone and progestational compounds attenuate tumor necrosis factor alpha-induced interleukin-8 production via nuclear factor kappa B inactivation in endometriotic stromal cells. Fertil Steril. 2005;83(5):1530–1535

    Article  CAS  PubMed  Google Scholar 

  32. Wieser F, Vigne JL, Ryan I, Hornung D, Djalali S, Taylor RN. Sulindac suppresses nuclear factor-kappaB activation and RANTES gene and protein expression in endometrial stromal cells from women with endometriosis. J Clin Endocrinol Metabol. 2005;90(12):6441–6447

    Article  CAS  Google Scholar 

  33. Kuroda K, Kitade M, Kikuchi I, et al. Peritoneal vascular density assessment using narrow-band imaging and vascular analysis software, and cytokine analysis in women with and without endometriosis. J Minim Invasive Gynecol. 2009;3(1):704–710

    Google Scholar 

  34. Kyama CM, Overbergh L, Mihalyi A, et al. Endometrial and peritoneal expression of aromatase, cytokines, and adhesion factors in women with endometriosis. Fertil Steril. 2008;89(2):301–310

    Article  CAS  PubMed  Google Scholar 

  35. Chen Q, Zhou W, Pu D, Li Z, Huang Q, Chen Q. The inhibitory effect of 15-R-LXA4 on experimental endometriosis. Eur J Obstet Gynecol Reprod Biol. 2009;145(2):200–204

    Article  CAS  PubMed  Google Scholar 

  36. Chen QH, Zhou WD, Su ZY, Huang QS, Jiang JN, Chen QX. Change of proinflammatory cytokines follows certain patterns after induction of endometriosis in a mouse model. Fertil Steril. 2009;31(5):217–224

    CAS  Google Scholar 

  37. Gogacz M, Bogusiewicz M, Putowski L, et al. Expression of tumor necrosis factor-alpha (TNF-alpha) on peritoneal fluid mononuclear cells in women with endometriosis. Ginekol Pol. 2008;79(1):31–35

    PubMed  Google Scholar 

  38. Kim HO, Yang KM, Kang IS, et al. Expression of CD44s, vascular endothelial growth factor, matrix metalloproteinase-2 and Ki-67 in peritoneal, rectovaginal and ovarian endometriosis. J Reprod Med. 2007;52(3):207–213

    CAS  PubMed  Google Scholar 

  39. Park JS, Lee JH, Kim M, Chang HJ, Hwang KJ, Chang KH. Endometrium from women with endometriosis shows increased proliferation activity. Fertil Steril. 2009;92(4):1246–1249

    Article  CAS  PubMed  Google Scholar 

  40. Celik O, Hascalik S, Elter K, Tagluk ME, Gurates B, Aydin NE. Combating endometriosis by blocking proteasome and nuclear factor-kappaB pathways. Hum Reprod. 2008;23(11):2458–2465

    Article  CAS  PubMed  Google Scholar 

  41. Cimşit M, Uzun G, Yildiz S. Hyperbaric oxygen therapy as an anti-infective agent. Expert Rev Anti Infect Ther. 2009;7(8):1015–1026

    Article  PubMed  Google Scholar 

  42. Bessereau J, Tabah A, Genotelle N, Français A, Coulange M, Annane D. Middle-ear barotrauma after hyperbaric oxygen therapy. Undersea Hyperb Med. 2010;37(4):203–208

    PubMed  Google Scholar 

  43. Lee CH, Chen WC, Wu CI, Hsia TC. Tension pneumocephalus: a rare complication after hyperbaric oxygen therapy. Am J Emerg Med. 2009;27(2):251–257

    Article  CAS  Google Scholar 

  44. Rivalland G, Mitchell SJ, van Schalkwyk JM. Pulmonary barotrauma and cerebral arterial gas embolism during hyperbaric oxygen therapy. Aviat Space Environ Med. 2010;81(9):888–890

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology of Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey

    Yavuz Aydin

  2. Department of Obstetrics and Gynecology, Sisli Etfal Training and Research Hospital, Istanbul, Turkey

    Alev Atis & Hicran Acar

  3. Obstetrics and Gynecology Department, Cerrahpasa Medical Faculty of Istanbul University, Istanbul, Turkey

    Seyfettin Uludag

  4. Department of Underwater and Hyperbaric Medicine, Istanbul University, Istanbul, Turkey

    Irem Tezer & Akin Toklu

  5. Hystopathology Department, Sisli Etfal Training and Research Hospital, Istanbul, Turkey

    Damlanur Sakiz

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  1. Yavuz Aydin
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Correspondence to Yavuz Aydin.

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Aydin, Y., Atis, A., Uludag, S. et al. Remission of Endometriosis by Hyperbaric Oxygen Treatment in Rats. Reprod. Sci. 18, 941–947 (2011). https://doi.org/10.1177/1933719111400635

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