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Variation in Hyaluronan-Binding Protein 2 (HABP2) Promoter Region is Associated With Unexplained Female Infertility

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Abstract

We set up to analyze polymorphisms in hyaluronan-binding protein 2 (HABP2) gene in healthy fertile women (n = 158) and in women with unexplained infertility (n = 116) and to investigate the potential role of HABP2 in receptive endometrium. Minor rs1157916 A and the major rs2240879 A alleles together with AA genotypes were significantly less frequent in infertile women than in controls. Immunohistochemistry analysis of endometrial HABP2 expression at the time of implantation identified significantly lower HABP2 protein level in infertile women in stroma and vessels than in fertile women. Migration assay analysis of cultured trophoblast and endothelial cells toward HABP2 protein referred to the function of HABP2 in endometrial endothelial cells. In conclusion, our results indicate that polymorphisms in the regulatory region of HABP2 gene could influence gene expression levels in the receptive endometrium and could thereby be one reason for infertility complications in women with unexplained infertility. Additionally, HABP2 protein involvement in endometrial angiogenesis is proposed.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology K57, Department of Clinical Science, Intervention and Technology, Division of Obstetrics and Gynaecology, Karolinska Institutet, Karolinska University Hospital Huddinge, 14186, Stockholm, Sweden

    Signe Altmäe PhD

  2. Department of Women’s and Children’s Health, Uppsala University, Akademiska Sjukhuset, Uppsala, Sweden

    Theodora Kunovac Kallak MSc & Anneli Stavreus-Evers PhD

  3. Department of Women’s and Children’s Health, Obstetrics and Gynaecology, Karolinska Institutet, Stockholm, Sweden

    Barbo Fridén MD, PhD

Authors
  1. Signe Altmäe PhD
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  2. Theodora Kunovac Kallak MSc
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  3. Barbo Fridén MD, PhD
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  4. Anneli Stavreus-Evers PhD
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Corresponding author

Correspondence to Signe Altmäe PhD.

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Author’s Note

S.A. was involved in the study design, analysis and interpretation of the data, and drafting of the manuscript. T.K.K. contributed to the study design, analysis and interpretation of the data, and drafting of the manuscript. B.F. was involved in the sample collection and manuscript preparation. A.S-E. was involved in the study design, interpretation of the data and manuscript preparation.

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Altmäe, S., Kallak, T.K., Fridén, B. et al. Variation in Hyaluronan-Binding Protein 2 (HABP2) Promoter Region is Associated With Unexplained Female Infertility. Reprod. Sci. 18, 485–492 (2011). https://doi.org/10.1177/1933719110388849

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  • DOI: https://doi.org/10.1177/1933719110388849

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