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Impact of 2 Doses of Clorgyline on the Rat Preimplantation Embryo Development and the Monoamine Levels in Urine

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Abstract

We have evaluated the impact of chronic administration of clorgyline, a potent monoamine oxidase A inhibitor and a former antidepressant, on the preimplantation embryo development in Wistar rats. Females were injected intraperitoneally daily for 30 days with saline (control animals), or with a low-dose clorgyline (LDC, 0.1 mg/kg per d) or with a high-dose clorgyline (HDC, 1 mg/kg per d). Embryos were isolated on day 5 of pregnancy and urine was collected by puncture of the urinary bladder. The number of embryos per female did not differ between experimental groups and control, but we have recorded a decreased number of embryos in HDC group compared to LDC (P < .05). We have found that LDC significantly reduced the presence of healthy embryos and increased the presence of the degenerated embryos (P < .001). The administration of the LDC resulted in the lowest cell number in blastocysts. We have observed significantly increased serotonin levels in HDC group compared to both control (P < .05) and LDC animals (P < .01). Norepinephrine (NE) levels in both experimental groups were significantly elevated compared to controls. Dopamine levels did not differ between groups (P > .05). We speculate that lesser negative effect of HDC compared to LDC on the preimplantation embryo development could be the consequence of the lower NE levels and/or elevated serotonin levels. Potential mechanisms mediating clorgyline-induced impaired preimplantation embryo development are proposed.

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Authors and Affiliations

  1. Department of Anatomy, P. J. Šafárik University—Medical Faculty, Šrobárova 2, 040 01, Košice, Slovak Republic

    Jozef Mihalik PhD, DVM, Tatiana Špakovská MD, Ingrid Hodorová PhD, MD, Silvia Rybárová PhD, DVM & Katarína Schmidtová PhD, MD

  2. Department of Medical Chemistry and Biochemistry, P. J. Šafárik University—Medical Faculty, Košice, Slovak Republic

    Jana Mašlanková PhD, MSc, Mária Mareková PhD, MSc & Jaroslav Kušnír PhD, MSc

  3. Institute of Biology and Ecology, P. J. Šafárik University—Medical Faculty, Košice, Slovak Republic

    Peter Ferenc MSc

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  1. Jozef Mihalik PhD, DVM
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  2. Jana Mašlanková PhD, MSc
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  9. Katarína Schmidtová PhD, MD
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Correspondence to Jozef Mihalik PhD, DVM.

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Mihalik, J., Mašlanková, J., Špakovská, T. et al. Impact of 2 Doses of Clorgyline on the Rat Preimplantation Embryo Development and the Monoamine Levels in Urine. Reprod. Sci. 17, 734–741 (2010). https://doi.org/10.1177/1933719110369181

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