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Simulation of Intra-Amniotic Infection and the Fetal Inflammatory Response in a Novel Ex-vivo Human Umbilical Cord Perfusion Model

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Abstract

Objective

We aimed to design and test an ex-vivo human umbilical cord (HUC) perfusion model simulating a fetal inflammatory response (FIR) during intra-amniotic infection.

Study Design

A 2-chamber model was designed to accommodate 2 pieces of umbilical cord. Cord perfusion was performed with placental blood. Intra-amniotic exposure to infectious organisms was simulated by adding lipopolysaccharide to the artificial amniotic fluid (AAF) compartment. As a measure of inflammatory response, we used interleukin 8 (IL-8) concentration in AAF and plasma by enzyme-linked immunosorbent assay (ELISA). Wilcoxon signed rank test was used for statistical analysis.

Results

We established a stable physiological setup. Results revealed significantly elevated plasma IL-8 concentrations (n = 6, P < .05) in the blood compartment of umbilical cords exposed to lipopolysaccharide. Concentrations within AAF were not significantly elevated (n = 6, P = .3095).

Conclusion

Simulation of a FIR in an ex-vivo model of HUC perfusion under physiological conditions is possible. Further work is necessary to establish histological funisitis.

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Authors and Affiliations

  1. Pediatric Pulmonology and Neonatology, Hannover Medical School, Hannover, Germany

    Sarah Hassan, Christa Acevedo & Christiane E. L. Dammann MD

  2. Perinatal Neuroepidemiology Unit, Departments of Obstetrics and Pediatrics, Hannover Medical School, 30623, Hannover, Germany

    Wolfgang Bueter MD & Olaf Dammann MD

  3. Newborn Medicine, Floating Hospital for Children at Tufts Medical Center, Boston, MA, USA

    Christiane E. L. Dammann MD & Olaf Dammann MD

  4. Pediatric Clinical Research Center, Floating Hospital for Children at Tufts Medical Center, Boston, MA, USA

    Olaf Dammann MD

Authors
  1. Sarah Hassan
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  2. Wolfgang Bueter MD
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  3. Christa Acevedo
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  4. Christiane E. L. Dammann MD
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  5. Olaf Dammann MD
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Corresponding author

Correspondence to Wolfgang Bueter MD.

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Hassan, S., Bueter, W., Acevedo, C. et al. Simulation of Intra-Amniotic Infection and the Fetal Inflammatory Response in a Novel Ex-vivo Human Umbilical Cord Perfusion Model. Reprod. Sci. 17, 426–433 (2010). https://doi.org/10.1177/1933719110361386

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  • DOI: https://doi.org/10.1177/1933719110361386

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