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In Vitro Models to Study the Pathogenesis of Endometriosis

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Abstract

Several in vitro models that attempt to replicate the intraperitoneal environment have been developed to study the pathogenesis of endometriosis. The chicken chorioallantotic membrane has been used, but it has not been well characterized and may introduce some species specific variables. In vitro models using human tissues include amniotic membrane, human peritoneal explants, and cell culture monolayers. These models have been used to qualitatively, quantitatively, and temporally assess attachment of endometrial cells to peritoneal mesothelial and subsequent transmesothelial invasion. These models have also been used to assess the role of cytokines in the development of the early endometriotic lesion. Two- and three dimensional invasion chamber models have been utilized to assess endometrial cell interactions with peritoneal mesothelial cells and the extracellular matrix. Invasion models are also useful to evaluate novel therapeutic approaches. This review will focus on the above models to assist reproductive scientists interested in the pathogenesis of endometriosis.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, University of Texas Health Science Center San Antonio, 7703 Floyd Curl Dr. Mail Code: 7836, San Antonio, Texas, 78229, USA

    Jason S. Griffith MD, Allison K. Rodgers MD & Robert S. Schenken MD

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  1. Jason S. Griffith MD
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  2. Allison K. Rodgers MD
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  3. Robert S. Schenken MD
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Correspondence to Robert S. Schenken MD.

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Griffith, J.S., Rodgers, A.K. & Schenken, R.S. In Vitro Models to Study the Pathogenesis of Endometriosis. Reprod. Sci. 17, 5–12 (2010). https://doi.org/10.1177/1933719109338221

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