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p16INK4A Is a Strong Biomarker for Cervical Intraepithelial Neoplasia and Invasive Cervical Carcinoma: A Reappraisal

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Abstract

To assess the role of p16INK4A, bcl–2, and p53 in cervical cancer screening, we conducted a retrospective trial of prospectively collected data. Sixty two women with abnormal Pap smears were subjected to colposcopy and biopsies from any abnormal lesion. Human papillomavirus (HPV)-DNA typing, histology, and immunochemistry for p16INK4A, bcl-2, and p53 were performed or these women. Histologic diagnosis was that of low grade squamous intraepithelial lesion (LSIL), high grade squamous intraepithelial lesion (HSIL), or cancer in all cases. Human papillomavirus strains were identified in 56 patients (90.3%). All HSIL and invasive cancer cases were HPVpositive. p16INK4A immunostaining yielded 100% sensitivity, 76% specificity, 61% positive predictive value, and 100% negative predictive value in cancer patients. The corresponding peformance indicators forHSIL patients were 75%, 62%, 32%, and 91%. bcl-2 and p53 expression did not correlate with worsening grades of cervical disease. We conclude that p16INK4A seems to be a sensitive biomarker o high grade cervical intraepithelial neoplasia and cancer.

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Authors and Affiliations

  1. Departments of Obstetrics and Gynecology and Pathology, “Alexandra” Hospital, University of Athens, Medical School, 80 Vasilissis Sofias Av., 11528, Greece, Athens, USA

    Dimitrios Haidopoulos MD, George A. Partsinevelos MD, George D. Vlachos MD, Alexandros Rodolakis MD, Sonia Markaki MD, Zannis Voulgaris MD, Emmanuel Diakomanolis PhD & Aris Antsaklis PhD

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  1. Dimitrios Haidopoulos MD
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  2. George A. Partsinevelos MD
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  3. George D. Vlachos MD
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  4. Alexandros Rodolakis MD
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  5. Sonia Markaki MD
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  6. Zannis Voulgaris MD
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Correspondence to George A. Partsinevelos MD.

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Haidopoulos, D., Partsinevelos, G.A., Vlachos, G.D. et al. p16INK4A Is a Strong Biomarker for Cervical Intraepithelial Neoplasia and Invasive Cervical Carcinoma: A Reappraisal. Reprod. Sci. 16, 685–693 (2009). https://doi.org/10.1177/1933719109334259

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