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PP13 mRNA Expression in Trophoblasts From Preeclamptic Placentas

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Abstract

To assess the role of placental protein 13 (PP13) in the pathogenesis of preeclampsia, the messenger RNA expression of PP13 was investigated in the trophoblasts from first and third trimester placenta. First, syncytiotrophoblasts and extravillous trophoblasts were separated from frozen section of third trimester placenta obtained from pregnant women with and without preeclampsia by laser microdissection. The PP13 levels of syncytiotrophoblasts were significantly higher than those of extravillous trophoblasts. The expression levels in syncytiotrophoblasts from the preeclampsia were significantly lower than those from normal. Next, the PP13 expression was assessed in trophoblasts from residual samples of chorionic villus sampling at 11 weeks of gestation, who subsequently developed preeclampsia. The levels in the preeclampsia group were significantly lower than those of normal cases. These findings indicate that an alteration in the PP13-messenger RNA expression in the trophoblasts may be associated with the pathogenesis of preeclampsia.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Showa University School of Medicine, Tokyo, Japan

    Akihiko Sekizawa MD, Shiho Yoshimura MD, Masamitsu Nakamura MD, Hanako Shimizu MD & Takashi Okai MD

  2. Department of Obstetrics and Gynecology, University of Indonesia, Indonesia

    Yuditiya Purwosunu MD

  3. Division of Prenatal Medicine Department of Histology and Embryology, University of Bologna, Bologna, Italy

    Nicola Rizzo MD & Antonio Farina MD

Authors
  1. Akihiko Sekizawa MD
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  2. Yuditiya Purwosunu MD
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  3. Shiho Yoshimura MD
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  4. Masamitsu Nakamura MD
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  5. Hanako Shimizu MD
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  6. Takashi Okai MD
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  7. Nicola Rizzo MD
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  8. Antonio Farina MD
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Correspondence to Akihiko Sekizawa MD.

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Sekizawa, A., Purwosunu, Y., Yoshimura, S. et al. PP13 mRNA Expression in Trophoblasts From Preeclamptic Placentas. Reprod. Sci. 16, 408–413 (2009). https://doi.org/10.1177/1933719108328615

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  • DOI: https://doi.org/10.1177/1933719108328615

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