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Mechanisms of Leukocyte Accumulation and Activation in Chorioamnionitis: Interleukin 1β and Tumor Necrosis Factor Α Enhance Colony Stimulating Factor 2 Expression in Term Decidua

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Abstract

Chorioamnionitis is a major cause of prematurity as well as perinatal morbidity and mortality. The present study observed a marked increase in immunohisto chemical staining for Colony Stimulating Factor 2 (CSF2; also known as granulocyte macrophage-colony stimulating factor), a potent neutrophil and macrophage chemoattractant and activator, in the decidua of patients with CAM compared with controls (n = 8; P = .001). To examine the regulation of this CSF2, cultured decidual cells primed with estradiol (E2) or E2 plus medroxyprogesterone acetate, were exposed to tumor necrosis factor-a or interleukin–1β and secreted CSF2 measured by ELISA. Levels of CSF2 in E2 plus MPA-treated cultures increased 18- and 245-fold following treatment with TNF or IL1B (n = 7, P < .05). Quantitative RT-PCR demonstrated parallel changes in mRNA levels. This study reveals that CSF2 is strongly expressed in decidua from patients with CAM and indicates TNF or IL1B as important regulators of CAM-related decidual leukocyte infiltration and activation.

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Authors and Affiliations

  1. Department of Human Pathology and Oncology, University of Siena, Italy

    Felice Arcuri PhD, Paolo Toti MD, Alessandra Casciaro MS & Marcella Cintorino MD

  2. Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, 333 Cedar Street, 208063/LCI room 810, 06520, CT, New Haven, USA

    Lynn Buchwalder MS, Frederick Schatz PhD & Charles J. Lockwood MD

  3. Department of Molecular and Cellular Biology, Harvard University, Cambridge, UK

    Basya Rybalov PhD

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  1. Felice Arcuri PhD
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Correspondence to Frederick Schatz PhD.

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Arcuri, F., Toti, P., Buchwalder, L. et al. Mechanisms of Leukocyte Accumulation and Activation in Chorioamnionitis: Interleukin 1β and Tumor Necrosis Factor Α Enhance Colony Stimulating Factor 2 Expression in Term Decidua. Reprod. Sci. 16, 453–461 (2009). https://doi.org/10.1177/1933719108328609

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