Abstract
Objective: Acute effects of ovulation, the tumor suppressor disruptor dimethylbenzanthracene, the mitogen estradiol-17β, and the antioxidant vitamin E on the ovarian epithelium were assessed in seasonally anestrous ewes. Methods: Dimethylbenzanthracene and vitamin E were administered before and estradiol after gonadotropin-induced ovulation. Ovaries were removed for histopathology one and three weeks following ovulation. Surface epithelial cells isolated at one week were immunostained for 8-oxoguanine and assayed for urokinase plasminogen activator secretion—markers of genotoxic and invasive potentials. Results: Ovarian surface invaginations and cortical inclusion cysts containing stratified epithelium were observed at three weeks in ovulated animals treated with dimethylbenzanthracene and estradiol. Progenitor cells were 8-oxoguanine positive and hypersecreted urokinase plasminogen activator. Untoward responses were circumvented by vitamin E. Conclusion: Antioxidant therapy appears to be of value in precluding periovulatory disturbances to the ovarian surface epithelium that has been associated with carcinogenesis.
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Murdoch, W.J., Van Kirk, E.A. & Shen, Y. Pathogenic Reactions of the Ovarian Surface Epithelium to Ovulation, Dimethylbenzanthracene, and Estrogen are Negated by Vitamin E. Reprod. Sci. 15, 839–845 (2008). https://doi.org/10.1177/1933719108322435
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DOI: https://doi.org/10.1177/1933719108322435