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Human Nonmetastatic Clone 23 Type 1 Gene Suppresses Migration of Cervical Cancer Cells and Enhances the Migration Inhibition of Fungal Immunomodulatory Protein From Ganoderma tsugae

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Abstract

The authors investigate the effects of human nonmetastatic clone 23 type 1 (nm23-H1) gene and fungal immunomodulatory protein—Ganoderma tsugae (FIP-gts) on the metastatic potential of cervical cancer cells and assess whether nm23-H1 can influence the action of FIP-gts using cell migration and invasion assays and gelatin zymography. The nm23-H1 gene was stably transfected into Caski cells, which lacked nm23-H1 expression. The results show that nm23-H1 stably transfected Caski cells exhibit reduced cell migration but no change of cell invasion and matrix metalloproteinase (MMP)—2 and —9 activities. FIP- gts reduced cell migration in SiHa and nm23-H1 transfected Caski cells more significantly compared with Caski cells and reduced invasion in Caski and nm23-H1—transfected Caski cells, but it exerted no influence on MMP-2 and MMP-9 activities in them. Conclusively, the nm23-H1 gene suppresses cervical cancer cell migration but not invasion and activities of MMP-2 and MMP-9 and enhances the inhibition of FIP-gts upon migration.

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Author notes

  1. L-YL and J-LK contributed equally to this work.

Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Chung Shan Medical University Hospital, Taichung, Taiwan

    Po-Hui Wang MD, PhD, Gin-Den Chen MD & Long-Yau Lin MD, PhD

  2. Clinical Research Center, Chung Shan Medical University Hospital, Taichung, Taiwan

    Chih-Ping Han MD, PhD

  3. Department of Family Medicine, Chung Shan Medical University Hospital, Taichung, Taiwan

    Shiuan-Chih Chen MD, PhD

  4. School of Medicine, Chung Shan Medical University, Taichung, Taiwan

    Po-Hui Wang MD, PhD, Gin-Den Chen MD & Long-Yau Lin MD, PhD

  5. Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan

    Po-Hui Wang MD, PhD, Shun-Fa Yang PhD, Chih-Ping Han MD, PhD & Long-Yau Lin MD, PhD

  6. Department of Pathology, Chung Shan Medical University, Taichung, Taiwan

    Chih-Ping Han MD, PhD

  7. Institute of Medical and Molecular Toxicology, Chung Shan Medical University, 110, Section 1, Chien-Kuo North Road, Taichung, 40201, Taiwan

    Jiunn-Liang Ko PhD

Authors
  1. Po-Hui Wang MD, PhD
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  2. Shun-Fa Yang PhD
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  3. Gin-Den Chen MD
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  4. Chih-Ping Han MD, PhD
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  5. Shiuan-Chih Chen MD, PhD
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Corresponding author

Correspondence to Jiunn-Liang Ko PhD.

Additional information

Grant support was provided by Chung Shan Medical University (CSMU 95-OM-B-014). The authors have no conflicts of interest.

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Wang, PH., Yang, SF., Chen, GD. et al. Human Nonmetastatic Clone 23 Type 1 Gene Suppresses Migration of Cervical Cancer Cells and Enhances the Migration Inhibition of Fungal Immunomodulatory Protein From Ganoderma tsugae. Reprod. Sci. 14, 475–485 (2007). https://doi.org/10.1177/1933719107305035

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  • DOI: https://doi.org/10.1177/1933719107305035

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