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Trichostatin A, a Histone Deacetylase Inhibitor, Attenuates Invasiveness and Reactivates E-Cadherin Expression in Immortalized Endometriotic Cells

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Abstract

The objective of this study is to determine whether trichostatin A (TSA) can suppress the invasiveness of 2 endometriotic cell lines known to be invasive and E-cadherin negative. The membrane invasion culture system was used to assess cell invasion using invasive and a noninvasive bladder cancer cell lines as positive and negative controls, respectively. The E-cadherin mRNA levels and protein expression were evaluated by real-time reverse transcriptase polymerase chain reaction and Western blot analysis, respectively. The authors found that TSA attenuates the invasiveness of 2 cell lines in the presence or absence of tumor necrosis factor α (TNFα) stimulation. In addition, TSA treatment reactivates E-cadherin gene and protein expression in these cell lines. These results, along with recent findings that TSA suppresses proliferation, interleukin-1β—induced cyclo-oxygenase 2 expression, and constitutive or TNFα-stimulated nuclear factor κ B activation in endometrial and endometriotic cells, makes histone deacetylase inhibitors a promising class of compounds for novel and more effective medical treatment of endometriosis, especially given the mounting evidence that endometrios be an epigenetic disease.

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Authors and Affiliations

  1. Department of Pediatrics, Medical College of Wisconsin, 8701 Watertown Plank Road, MS 756, Milwaukee, WI, 53226-0509, USA

    Yan Wu PhD & Sun-Wei Guo PhD

  2. Molecular Cell Biology and Human Genetics in Biosciences, Johann Wolfgang Goethe University, Frankfurt/Main, Germany

    Anna Starzinski-Powitz PhD

Authors
  1. Yan Wu PhD
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  2. Anna Starzinski-Powitz PhD
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  3. Sun-Wei Guo PhD
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Correspondence to Sun-Wei Guo PhD.

Additional information

Financial support was provided by The Children’s Research Institute, Milwaukee, Wisconsin. We thank Alexander Schreiner for critical reading of the article, Monika Kamprad for expert technical assistance, and 2 anonymous reviewers for their constructive comments on an earlier version of the article.

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Wu, Y., Starzinski-Powitz, A. & Guo, SW. Trichostatin A, a Histone Deacetylase Inhibitor, Attenuates Invasiveness and Reactivates E-Cadherin Expression in Immortalized Endometriotic Cells. Reprod. Sci. 14, 374–382 (2007). https://doi.org/10.1177/1933719107302913

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