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Inhibitory Effect of Interleukin-8 on the Secretion of Platelet-Activating Factor Acetylhydrolase by Human Decidual Macrophages

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The Journal of the Society for Gynecologic Investigation: JSGI Aims and scope Submit manuscript

Abstract

Objective

To determine whether interleukin-8 (IL-8) regulates platelet-activating factor acetylhydrolase (PAF-AH) secretion by decidual cells in vitro.

Methods

Decidual macrophages were obtained from human term decidual tissue by enzymic digestion and Ficoll-Paque centrifugation. The effect of IL-8 and phorbol esters on the secretion of PAF-acetylhydrolase by these cells was examined.

Results

IL-8 inhibited PAF-AH secretion by decidual macrophages in a dose-dependent manner. The inhibition was reversed partially by antibodies against tumor necrosis factor-α and IL-1β. IL-8-induced inhibition was blocked partially by the protein kinase C (PKC) inhibitors, sphingosine, and H-7 1-(5-isoquinoline sulfonyl)-2-methylpiperazine. A PKC activator, 12-O-tetradecanoyl phorbol 13-acetate, decreased PAF-AH secretion.

Conclusion

IL-8 may increase the PAF concentration in the decidua via its inhibitory effect on PAF-AH secretion by decidual macrophages. IL-8-induced inhibition of enzyme secretion may have been mediated in part by PKC-dependent signal transduction.

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Authors and Affiliations

  1. Departments of Biochemistry and Obstetrics and Gynecology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, 75235-9051, Dallas, Texas, USA

    Yasushi Kawano MD, Hisashi Narahara MD & John M. Johnston PhD

Authors
  1. Yasushi Kawano MD
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  2. Hisashi Narahara MD
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  3. John M. Johnston PhD
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Corresponding author

Correspondence to John M. Johnston PhD.

Additional information

Supported in part by the Chilton Foundation, Dallas, Texas.

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Kawano, Y., Narahara, H. & Johnston, J.M. Inhibitory Effect of Interleukin-8 on the Secretion of Platelet-Activating Factor Acetylhydrolase by Human Decidual Macrophages. Reprod. Sci. 6, 328–332 (1999). https://doi.org/10.1177/107155769900600609

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