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Expression of HOXDIO Gene in Normal Endometrium and Endometrial Adenocarcinoma

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Abstract

Objective

Hox genes encode DNA transcription regulatory that contain a conserved 61 amino acid protein called the homeodomain. Although best known for their role in cellular differentiation during embryonic development, aberrant expression of these genes has been associated with hematologic and solid neoplasms. The purpose of this study was to determine the relative expression of HOXD10 in huamn endometrial adenocarcionmas.

Methods

mRNA was isolated from 7 normal endometrial specimens and 28 endometrial adenocarcinoma specimens. cDNA was synthesized using randon hexamer primers. The expression of HOXD10 relative to βtubulin (internal control) was assessed by densitometric comparison of co-amplified Phosphorus-32 (32P) labeled gene products separated by agarose gel electrophoesis. Direct sequencing of purified HOXD10 polymerase chain reaction product was also performed.

Results

The sequence of the purified HOXD10 product corresponds to the known DNA sequence reported in the National Institutes of Health Gene Bank. mRNA expression of HOXD10 relative to β-tubulin is significantly lower in endometrial carcinomas than in normal endometrium. Furthermore, the ratio of HOXD10 to β-tubulin expression varies inversely with the histologic grade of the tumor (P = .0009).

Conclusion

Cancer is a multistep proces involving aberrant expression of genes that regulate cell growth and differentiation. Human HOXD10 gene expression is altered in endometrial carcinoma and varies with the histologic grade of differentiation. This observation supports the theory that homeobox genes play a role in oncogenesis.

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Authors and Affiliations

  1. Division of Gynecologic Oncology, University of Michigan, Ann Arbor, Michigan, USA

    Janet Osborne MD, Changzi Hu MD, Colleen Hawley BS, Lowell J. Underwood BS, Timothy J. O’Brien PhD & Vicki V. Baker MD

  2. Departments of Obsteries and Gynecology, and Biocgemistry and Moclecular Biology, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA

    Janet Osborne MD, Changzi Hu MD, Colleen Hawley BS, Lowell J. Underwood BS, Timothy J. O’Brien PhD & Vicki V. Baker MD

Authors
  1. Janet Osborne MD
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  2. Changzi Hu MD
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  3. Colleen Hawley BS
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  4. Lowell J. Underwood BS
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  5. Timothy J. O’Brien PhD
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  6. Vicki V. Baker MD
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Additional information

Funded in part by the American Cancer Society Clinical Oncology Fellowship Award, grant 95–781.

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Osborne, J., Hu, C., Hawley, C. et al. Expression of HOXDIO Gene in Normal Endometrium and Endometrial Adenocarcinoma. Reprod. Sci. 5, 277–280 (1998). https://doi.org/10.1177/107155769800500509

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