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Red Cell Volume Determination Using a Stable Isotope of Chromium

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The Journal of the Society for Gynecologic Investigation: JSGI Aims and scope Submit manuscript

Abstract

Objective

To validate and improve a method of red cell volume determination by use of a stable isotope of chromium.

Methods

Twelve subjects were sequentially injected with red blood cells labeled with a stable isotope of chromium (53Cr) and red blood cells labeled with radioisotopic chromium (51Cr). Measurement of 53Cr dilution was by gas chromatography/mass spectrometry. Measurement of 51Cr dilution was by gamma counter.

Results

Comparison of the two methods led to results that differed on average by 34.5 ± 45.0 mL (1.8 ± 2.2%), 0.3 to 3.2%, 95% confidence interval.

Conclusion

Measurement of red cell volume by use of a stable isotope of chromium is accurate, with potential applications including measurement in pregnant women and children or other groups in whom exposure to radioisotopes is undesirable.

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Authors and Affiliations

  1. Departments of Obstetrics and Gynecology and Pediatrics, Brown University, Women and Infants’ Hospital of Rhode Island, Providence, Rhode Island, USA

    Helayne M. Silver MD, Mary Ann Seebeck RN, Richard M. Cowett MD, Kristine Y. Patterson PhD & Claude Veillon PhD

  2. Human Nutrition Research Center, U.S. Department of Agriculture, Beltsville, Maryland, USA

    Helayne M. Silver MD, Mary Ann Seebeck RN, Richard M. Cowett MD, Kristine Y. Patterson PhD & Claude Veillon PhD

Authors
  1. Helayne M. Silver MD
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  2. Mary Ann Seebeck RN
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  3. Richard M. Cowett MD
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  4. Kristine Y. Patterson PhD
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  5. Claude Veillon PhD
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Silver, H.M., Seebeck, M.A., Cowett, R.M. et al. Red Cell Volume Determination Using a Stable Isotope of Chromium. Reprod. Sci. 4, 254–258 (1997). https://doi.org/10.1177/107155769700400506

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  • DOI: https://doi.org/10.1177/107155769700400506

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