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Developmental Changes in Renin-Containing Cells From the Ovine Fetal Kidney

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Abstract

Objective

To determine if the increase in intrarenal renin content that occurs in late gestation in the fetal lamb results from an increase in the proportion of cells containing renin, an increase in the amount of renin per renin-containing cell, or both.

Methods

Kidney cells with a density of 1.062 g/mL were isolated from ovine fetuses at 100-104 days gestation (n = 6, immature) and at 131—139 days gestation (n = 6, mature). This fraction was enriched with renin-containing cells. Total renin (TR) and active renin (AR) were measured by their ability to generate angiotensin I (AI). Renin mRNA was measured by RNase protection assay. The presence of renin in cells and the capacity to release it were identified by immunocytochemistry and cell immunoblot analyses. Data were analyzed by analyses of variance.

Results

The TR and AR in isolated cells increased from 3.7 ± 0.3 and 2.5 ± 0.2 μg AI/μg protein · hr-1 in immature fetuses to 6.2 ± 0.7 and 5.0 ± 0.6 μg AI/gmg protein • hr-1 in mature fetuses (P <.01). The percentage of immunopositive renin cells (PC) was greater in mature (40.6 ± 4.3) than in immature fetuses (15.2 ± 1.2) (P <.01). The TR/PC and AR/PC were also higher in mature (8.2 ±0.6 and 6.6 ± 0.4 ng AI/h • pc-1) than in immature animals (6.5 ±0.6 and 4.4 ± 0.4 ng AI/h · pc-1) (P <.01).

Conclusions

The data indicate that the increase in TR and AR occurring during development in fetal lamb kidney results from an increase in both the number of renin-containing cells and in the renin content per cell. (J Soc Gynecol Invest 1997;4:191-196) Copyright © 1997 by the Society for Gynecologic Investigation.

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Authors and Affiliations

  1. Departments of Physiology and Pharmacology and Obstetrics and Gynecology and the Perinatal Research Laboratories, Bowman Gray School of Medicine of Wake Forest University, Medical Center Boulevard, Winston-Salem, NC, 27157, USA

    Jinjuan Wang MD, Frank M. Perez PhD & James C. Rose PhD

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  1. Jinjuan Wang MD
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  2. Frank M. Perez PhD
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  3. James C. Rose PhD
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Additional information

Supported by National Institutes of Health* grant no. HD17644.

We thank Dr. Jeffrey Schwartz for his insightful comments about the manuscript. Dr. Eberhard Mueller-Heubach for his support and Linda Trust, Portia Ellerbe and Donna Jones for expert secretarial assistance.

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Wang, J., Perez, F.M. & Rose, J.C. Developmental Changes in Renin-Containing Cells From the Ovine Fetal Kidney. Reprod. Sci. 4, 191–196 (1997). https://doi.org/10.1177/107155769700400404

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