Abstract
Objective
TO test the hypothesis that interleukin-1β (IL- 1β) and tumor necrosis factor-α (TNF-α) regulate granulocyte colony-stimulating factor (G-CSF) production by human placental villous core mesenchymal cells.
Methods
Villous core mesenchymal cells were isolated from placentas at 14—20 weeks’ gestation and cultured in vitro. Cells were treated with IL-1β or TNF-α in dose-response and time-course studies. We measured G-CSF mRNA expression by Northern blot analysis and G-CSF protein production by enzyme-linked immunosorbent assay of the conditioned media.
Results
Unstimulated mesenchymal cells expressed negligible G-CSF. Steady-state G-CSF mRNA expression was maximal 3–6 hours after IL- 1β treatment and 6–18 hours after TNF-α treatment. Each cytokine induced G-CSF protein production in dose- and time-dependent manners, with IL-1β more potent than TNF-α. The G-CSF mRNA expression and G-CSF protein production induced by the combination of both cytokines exceeded that induced by either cytokine alone.
Conclusions
Interleukin-1β and TNF-α stimulate G-CSF production by placental villous core mesenchymal cells in vitro. These results identify a potential mechanism by which villous core mesenchymal cells mediate, in part, the placental response to these two cytokines. (J Soc Gynecol Invest 1996;3:172-8)
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This work was supported in parr by A. D. Williams Fund Grant 6-6746 (DTV) and National Institutes of Health grant HD29023 (SWK).
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Vandermolen, D.T., Kauma, S.W. & Turner, T.T. Interleukin-1β and Tumor Necrosis Factor-α Stimulate Granulocyte Colony-Stimulating Factor Production by Placental Villous Core Mesenchymal Cells. Reprod. Sci. 3, 172–178 (1996). https://doi.org/10.1177/107155769600300403
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DOI: https://doi.org/10.1177/107155769600300403