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Induction of Immunoreactive Prostaglandin H Synthases 1 and 2 and Fos in Response to Cerebral Hypoperfusion in Late-Gestation Fetal Sheep

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The Journal of the Society for Gynecologic Investigation: JSGI Aims and scope Submit manuscript

Abstract

Objective

The goal of the present study was to localize and quantify immunoreactive protaglandin H synthase 1 (PGHS-1), PGHS-2, and Fos expression by immunohistochemistry in the fetal brain 30 minutes and 2 hours after the onset of a 10-minute period of cerebral hypoperfusion in barodenervated and chemodenervated fetal sheep.

Methods

Fetal sheep of known gestational age were studied intact or sinoaortic denervated. Fetuses were sacrificed and tissues recovered for immunohistochemistry or real-time polymerase chain reaction measurements of protein and mRNA, respectively. Some fetuses were subjected to brachiocephalic occlusion, produced by inflation of an extravascular balloon occluder around the brachiocephalic artery. Immunohistochemistry results were quantified using image analysis, and mRNA was quantified by estimation of cycle threshold in generation of PGHS-1 or PGHS-2 amplicons.

Results

Sinoaortic denervation by itself did not alter the abundance of PGHS-1 or PGHS-2 protein in any brain region, although the denervation did reduce the abundance of PGHS-1 mRNA in hypothalamus. We assessed PGHS-1, PGHS-2, and Fos immunoreactive protein abundance by image analysis of histologic sections stained for the respective proteins using immunohistochemistry. Cerebral hypoperfusion increased the intensity of staining of immunoreactive PGHS-1, PGHS-2, and Fos in the anterior pituitary, hippocampus, and cerebellum. In the cerebral microvasculature, the intensity of PGHS-1 and Fos was significantly greater, and in the cerebral cortex, the intensity of PGHS-2 was significantly greater. Changes in the amount of immunostaining in the nucleus of tractus solitarius and paraventricular nucleus were not statistically significant.

Conclusion

Cerebral hypoperfusion altered the expression and distribution of prostaglandin biosynthetic enzymes in ovine fetal brain by a mechanism that is independent of baroreceptor and chemoreceptor afferent activity.

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Authors and Affiliations

  1. Department of Physiology and Functional Genomics, JHMHC, University of Florida College of Medicine, Box 100274, 32610-0274, Gainesville, Florida, USA

    Haiyan Tong MD, PhD, Kelley E. Gridley PhD & Charles E. Wood PhD

Authors
  1. Haiyan Tong MD, PhD
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  2. Kelley E. Gridley PhD
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  3. Charles E. Wood PhD
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Corresponding author

Correspondence to Charles E. Wood PhD.

Additional information

This work was supported by grant HD33053 from National Institute of Child Health and Human Development and a Grant-in-Aid from the Florida/Puerto Rico Affiliate of the American Heart Association to Dr. Wood, a predoctoral fellowship from the Florida/ Puerto Rico Affiliate of the American Heart Association to Dr. Tong, and a postdoctoral NRSA from the National Institute of Child Health and Human Development to Dr. Gridley.

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Tong, H., Gridley, K.E. & Wood, C.E. Induction of Immunoreactive Prostaglandin H Synthases 1 and 2 and Fos in Response to Cerebral Hypoperfusion in Late-Gestation Fetal Sheep. Reprod. Sci. 9, 342–350 (2002). https://doi.org/10.1177/107155760200900604

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