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Shiga Toxin 1 and 2 Induce Apoptosis in the Amniotic Cell Line WISH

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The Journal of the Society for Gynecologic Investigation: JSGI Aims and scope Submit manuscript

Abstract

Objective

The aim of this study was to evaluate the toxicity of Shiga toxin (Stx) 1 and 2 on amniotic cells in vitro.

Methods

WISH cells, which were derived from human amniotic cells, and Vero cells were cultured with or without Stxs. After 24 hours of culture, cell viability was measured by Cell Counting Kit-8, and extracted DNA was electrophoresed on a 1% agarose gel. The morphologic changes were observed by Papanicolaou staining, and the apoptotic index (percentage of apoptotic nuclei per total nuclei) was calculated. Quantification of apoptotic cells was also measured by an enzyme-linked immunosorbent assay.

Results

The viability of WISH cells decreased in proportion to the concentrations of Stxs. Cellular ladder formation was observed by DNA electrophoresis of Stx-treated WISH cells, and the typical morphologic changes were observed by Papanicolaou staining. The proportion of apoptotic cell increased in response to Stxs.

Conclusions

Stxs injured WISH cells directly and induced apoptosis in vitro. WISH cells were as sensitive as Vero cells to Stxs and cell death occurred by apoptosis.

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Authors and Affiliations

  1. Departments of Obstetrics and Gynecology, Microbiology, Pathology and Cell Biology, and Biochemistry, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, 807-8555, Kitakyushu, Japan

    Kazuaki Yoshimura MD, PhD, Akihide Tanimoto MD, PhD, Tetsuya Abe MD, PhD, Midori Ogawa PhD, Takashi Yutsudo PhD, Masamichi Kashimura MD, PhD & Shin-ichi Yoshida MD, PhD

  2. Shionogi Research Laboratories, Shionogi and Co Ltd., Osaka, Japan

    Kazuaki Yoshimura MD, PhD, Akihide Tanimoto MD, PhD, Tetsuya Abe MD, PhD, Midori Ogawa PhD, Takashi Yutsudo PhD, Masamichi Kashimura MD, PhD & Shin-ichi Yoshida MD, PhD

  3. Department of Bacteriology, Faculty of Medicine, Kyushu University, Fukuoka, Japan

    Kazuaki Yoshimura MD, PhD, Akihide Tanimoto MD, PhD, Tetsuya Abe MD, PhD, Midori Ogawa PhD, Takashi Yutsudo PhD, Masamichi Kashimura MD, PhD & Shin-ichi Yoshida MD, PhD

Authors
  1. Kazuaki Yoshimura MD, PhD
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  2. Akihide Tanimoto MD, PhD
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  3. Tetsuya Abe MD, PhD
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  4. Midori Ogawa PhD
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  5. Takashi Yutsudo PhD
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  6. Masamichi Kashimura MD, PhD
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  7. Shin-ichi Yoshida MD, PhD
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Corresponding author

Correspondence to Kazuaki Yoshimura MD, PhD.

Additional information

The authors thank Emmet Hirsch, Columbia University College of Physicians and Surgeons, for helpful comments and critical review of the manuscript.

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Yoshimura, K., Tanimoto, A., Abe, T. et al. Shiga Toxin 1 and 2 Induce Apoptosis in the Amniotic Cell Line WISH. Reprod. Sci. 9, 22–26 (2002). https://doi.org/10.1177/107155760200900105

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