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Importance of the Renal Nerves for Basal and Stimulated Renin mRNA Levels in Fetal and Adult Ovine Kidneys

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The Journal of the Society for Gynecologic Investigation: JSGI Aims and scope Submit manuscript

Abstract

Objective

To investigate the effect of renal denervation on renin mRNA levels in fetal and nonpregnant adult ovine renocortical tissue and in isolated juxtaglomerular cells under basal conditions and after stimulation.

Methods

The left kidney was denervated and the right kidney subjected to a sham procedure in nine ovine fetuses (136–41 days’ gestation) and 20 nonpregnant ewes. After 5–7 days the denervated and intact kidneys were obtained, and renin-containing renal cortical cells were isolated and cultured overnight. Then cells were treated with isoproterenol, forskolin, or isomethylbutyl xanthine (IBMX) for 4 hours. Total RNA was isolated and renin mRNA measured by RNase protection assay. Cyclic adenosine monophosphate (cAMP) levels were measured in the incubation medium with a competitive enzyme immunoassay.

Results

In adults, basal renin mRNA levels were significantly lower in denervated than in sham-operated kidneys. No diference was noted between denervated and intact fetal kidneys. Renin mRNA levels were significantly higher in fetal than in adult kidney tissue, and cells from fetuses had greater increases in renin mRNA after stimulation than did cells from adults. Fetal cells also released more cAMP into the incubation medium, and there was a correlation between cAMP and renin mRNA levels.

Conclusions

The data indicate the effects of renal denervation on renin mRNA expression in the kidney are age dependent and that thefetus in late gestation has a mechanism for maintaining renin mRNA levels after denervation, which is absent or nonfunctional in the adult.

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Authors and Affiliations

  1. Department of Physiology and Pharmacology, and Department of Obstetrics and Gynecology and Perinatal Research Laboratories, Wake Forest University School of Medicine, Medical Center Boulevard, Winston-Salem, North Carolina, 27157, USA

    Hiroyuki Ito MD, Jinjuan Wang MD, Jack W. Strandhoy PhD & James C. Rose PhD

Authors
  1. Hiroyuki Ito MD
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  2. Jinjuan Wang MD
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  3. Jack W. Strandhoy PhD
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  4. James C. Rose PhD
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Corresponding author

Correspondence to James C. Rose PhD.

Additional information

The authors acknowledge Mike Dunlap for technical expertise and Dr. Nancy Valego for continued support.

This work was supported by National Institutes of Health grant HD-17644 and an RJR-Leon Golberg Fellowship.

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Ito, H., Wang, J., Strandhoy, J.W. et al. Importance of the Renal Nerves for Basal and Stimulated Renin mRNA Levels in Fetal and Adult Ovine Kidneys. Reprod. Sci. 8, 327–333 (2001). https://doi.org/10.1177/107155760100800604

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