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Cyclin E mRNA Overexpression in Epithelial Ovarian Cancers: Inverse Correlation with p53 Protein Accumulation

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Abstract

Objective

We investigated the relationship between cyclin E mRNA overexpression and p53 protein accumulation in epithelial ovarian cancers.

Methods

mRNA was isolated and cDNA was prepared from 36 epithelial ovarian tumors (three adenomas, three low malignant potential tumors, and 30 carcinomas), and six normal ovaries. The cyclin E mRNA expression levels relative to an internal control, β-tubulin, were determined by semiquantitative polymerase chain reaction (PCR). Cyclin E and p53 protein expression in ovarian cancer tissues were examined by immunohistochemistry using the same series of samples. Fisher exact test of significance and an unpaired t test were used for statistical analysis.

Results

Considerable levels of cyclin E mRNA were detected in all normal ovaries and ovarian tumor samples examined by semiquantitative PCR amplification. mRNA levels of cyclin E were significantly higher in nine of 30 (30%) ovarian cancers compared with those in normal ovaries. The immunohistochemical expression of cyclin E protein was confirmed in the nuclei of tumor cells in 13 of 30 (43%) ovarian cancers. p53 protein accumulation was detected in 12 of 30 (40%) ovarian cancers examined. There was a significant inverse correlation between cyclin E mRNA overexpression and p53 protein accumulation (P <. 01, Fisher exact test).

Conclusions

Cyclin E mRNA overexpression frequently occurs in ovarian cancers without p53 protein accumulation. Cyclin E might have an important effect on the development of a limited number of ovarian cancers.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Kure National Hospital, Chugoku Cancer Center, Kure, Japan

    Takashi Sawasaki MD, Kazushi Shigemasa MD, Yuko Shiroyama MD, Tomoyo Kusuda MD, Tsuneo Fujii MD, Tim H. Parmley MD, Timothy J. O’Brien PhD & Koso Ohama MD

  2. Department of Obstetrics and Gynecology, Hiroshima University School of Medicine, 1-2-3, Kasumi, Minami-ku, 734-8551, Hiroshima, Japan

    Takashi Sawasaki MD, Kazushi Shigemasa MD, Yuko Shiroyama MD, Tomoyo Kusuda MD, Tsuneo Fujii MD, Tim H. Parmley MD, Timothy J. O’Brien PhD & Koso Ohama MD

  3. Departments of Obstetrics and Gynecology and Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA

    Takashi Sawasaki MD, Kazushi Shigemasa MD, Yuko Shiroyama MD, Tomoyo Kusuda MD, Tsuneo Fujii MD, Tim H. Parmley MD, Timothy J. O’Brien PhD & Koso Ohama MD

Authors
  1. Takashi Sawasaki MD
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  2. Kazushi Shigemasa MD
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  3. Yuko Shiroyama MD
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  4. Tomoyo Kusuda MD
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  5. Tsuneo Fujii MD
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  6. Tim H. Parmley MD
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  7. Timothy J. O’Brien PhD
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  8. Koso Ohama MD
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Corresponding author

Correspondence to Kazushi Shigemasa MD.

Additional information

This study was supported in part by a Grant-in-Aid for Cancer Research from the Ministry of Health and Welfare (No.10-12) and by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports, and Culture (No.12671605), Japan.

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Sawasaki, T., Shigemasa, K., Shiroyama, Y. et al. Cyclin E mRNA Overexpression in Epithelial Ovarian Cancers: Inverse Correlation with p53 Protein Accumulation. Reprod. Sci. 8, 179–185 (2001). https://doi.org/10.1177/107155760100800310

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  • DOI: https://doi.org/10.1177/107155760100800310

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