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Reproductive Sciences

, Volume 19, Issue 3, pp 306–311 | Cite as

Computer-Assisted Oocyte Morphometry Before ICSI: Correlation of Oocyte Measurements With Fertilization and Embryo Development

  • Maria das Graças R. S. Camargos
  • Veronica N. M. Lobach
  • Francisco A. N. Pereira
  • Cláudia N. C. D. Lemos
  • Fernando M. Reis
  • Aroldo F. CamargosEmail author
Original Articles

Abstract

The present study aimed to correlate morphometric parameters of the oocytes with the occurrence of fertilization following intracytoplasmic sperm injection (ICSI). In a prospective, controlled cohort design, women (n = 32) who were candidates for ICSI had oocytes (n = 258) collected and submitted to morphometric evaluation using the Cronus3 software program. The morphometric parameters obtained were oocyte diameter, perivitelline space width, zona pellucida thickness, and first polar body diameter. The median oocyte diameter was similar in cases in which fertilization occurred compared with those in which fertilization failed (75.2 and 75.9 μm, respectively; P = .218). The 2 groups also had similar measurements of perivitelline space, zona pellucida, and first polar body. However, the best quality zygotes identified by a morphological score resulted from oocytes with larger diameter (75.6 vs 74.0 μm; P < .01) and narrow perivitelline space (5.3 vs 7.1 μm; P < .01). Embryo development, as assessed by cleavage at second day of culture, was not significantly associated with oocyte morphometric parameters. These findings suggest that morphometric parameters of the oocytes do not correlate with the occurrence of fertilization following ICSI but may assist in selecting oocytes more likely to originate high-quality zygotes.

Keywords

oocyte morphometry ICSI morphology embryonic development 

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Copyright information

© Society for Reproductive Investigation 2012

Authors and Affiliations

  • Maria das Graças R. S. Camargos
    • 1
  • Veronica N. M. Lobach
    • 1
  • Francisco A. N. Pereira
    • 1
  • Cláudia N. C. D. Lemos
    • 1
  • Fernando M. Reis
    • 1
  • Aroldo F. Camargos
    • 1
    Email author
  1. 1.Laboratory of Human Reproduction, Hospital das Clínicas, and Department of Obstetrics and GynecologyFederal University of Minas GeraisBelo HorizonteBrazil

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