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Reproductive Sciences

, Volume 19, Issue 3, pp 290–297 | Cite as

Metabolism of 17-Alpha-Hydroxyprogesterone Caproate by Human Placental Mitochondria

  • Valentina M. Fokina
  • Olga L. Zharikova
  • Gary D. V. Hankins
  • Mahmoud S. Ahmed
  • Tatiana N. NanovskayaEmail author
Original Articles

Abstract

Perfusion of 17-alpha-hydroxyprogesterone caproate (17HPC) via the maternal circuit of a dually perfused human placental lobule resulted in the extensive formation of 2 metabolites. On the other hand, human placental microsomes biotransformed 17HPC into 5 monohydroxylated metabolites, which did not correspond to those formed during perfusion. The goal of this investigation was to determine the subcellular localization of the enzymes responsible for the biotransformation of 17HPC during its perfusion in human placenta. Crude subcellular fractions of the human placental tissue were utilized. Six 17HPC metabolites were formed by the placental mitochondrial fraction, of which 4 were identical to those formed by the microsomes; whereas the other 2, namely MM and M19, were formed by the mitochondrial fraction only. The latter metabolites were identical to those formed during 17HPC perfusion, as determined by liquid chromatography–mass spectrometry (LC-MS) analysis. Therefore, these data strongly suggest that the enzymes responsible for the biotransformation of 17HPC during its perfusion are predominantly localized in human placental mitochondria.

Keywords

17-alpha-hydroxyprogesterone caproate metabolism placenta mitochondria LC-MS 

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Copyright information

© Society for Reproductive Investigation 2012

Authors and Affiliations

  • Valentina M. Fokina
    • 1
  • Olga L. Zharikova
    • 1
  • Gary D. V. Hankins
    • 1
  • Mahmoud S. Ahmed
    • 1
  • Tatiana N. Nanovskaya
    • 1
    Email author
  1. 1.Department of Obstetrics and GynecologyUniversity of Texas Medical BranchGalvestonUSA

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