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Dystroglycan Expression in Human Placenta: Basement Membrane Localization and Subunit Distribution Change Between the First and Third Trimester

Abstract

Objectives

This study describes the distribution of dystroglycan (DG) in human placenta, membranes, and uterine decidua.

Study design

Dystroglycan expression was characterized by Western blotting, immunohistochemistry, and immunofluorescence microscopy using human tissues and cultured cells.

Results

Both α-DG and β-DG are expressed in the term syncytiotrophoblast, and α-DG is localized to the basement membrane. In first-trimester chorionic villi, α-DG and β-DG are localized to the periphery of the cytotrophoblast. Expression varies in term fetal membranes. α-Dystroglycan is not detectable in choriocarcinoma cells or HTR cells, but β-DG is present in both normal and cleaved forms.

Conclusion

Dystroglycan is expressed at high levels in human trophoblasts, and localization of the α- and β-subunits varies with gestational age and trophoblast differentiation. Because DG expression inversely correlates with invasiveness in many cancers, its pattern of expression in trophoblasts suggests a possible function in inhibition of placental invasion.

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References

  1. 1.

    Weir ML, Muschler J. Dystroglycan: emerging roles in mammary gland function. J Mammary Gland Biol Neoplasia. 2003;8(4): 409–419.

  2. 2.

    Barresi R, Campbell KP. Dystroglycan: from biosynthesis to pathogenesis of human disease. J Cell Sci. 2006;119(pt 2): 199–207.

  3. 3.

    Haenggi T, Fritschy JM. Role of dystrophin and utrophin for assembly and function of the dystrophin glycoprotein complex in non-muscle tissue. Cell Mol Life Sci. 2006;63(14):1614–1631.

  4. 4.

    Michele DE, Barresi R, Kanagawa M, et al. Post-translational disruption of dystroglycan-ligand interactions in congenital muscular dystrophies. Nature. 2002;418(6896):417–422.

  5. 5.

    Williamson RA, Henry MD, Daniels KJ, et al. Dystroglycan is essential for early embryonic development: disruption of Reichert’s membrane in Dag1-null mice. Hum Mol Genet. 1997;6(6):831–841.

  6. 6.

    Yotsumoto S, Fujiwara H, Horton JH, et al. Cloning and expression analyses of mouse dystroglycan gene: specific expression in maternal decidua at the peri-implantation stage. Hum Mol Genet. 1996;5(9): 1259–1267.

  7. 7.

    Anderson C, Winder SJ, Borycki AG. Dystroglycan protein distribution coincides with basement membranes and muscle differentiation during mouse embryogenesis. Dev Dyn. 2007;236(9):2627–2635.

  8. 8.

    Henry MD, Williamson RA, Campbell KP. Analysis of the role of dystroglycan in early postimplantation mouse development. Ann N Y Acad Sci. Oct. 1998;857:256–259.

  9. 9.

    Petroff MG, Phillips TA, Ka H, Pace JL, Hunt JS. Isolation and culture of term human trophoblast cells. Methods Mol Med. 2006;121(2):203–217.

  10. 10.

    Pereboev AV, Ahmed N, thi Man N, Morris GE. Epitopes in the interacting regions of beta-dystroglycan (PPxY motif) and dystrophin (WW domain). Biochim Biophys Acta. 2001;1527(1–2):54–60.

  11. 11.

    Zhou YW, Thomason DB, Gullberg D, Jarrett HW. Binding of laminin alpha1 -chain LG4–5 domain to alpha-dystroglycan causes tyrosine phosphorylation of syntrophin to initiate Rac1 signaling. Biochemistry. 2006;45(7):2042–2052.

  12. 12.

    Theiler RN, Compton T. Characterization of the signal peptide processing and membrane association of human cytomegalovirus glycoprotein O. J Biol Chem. 2001;276(42):39226–39231.

  13. 13.

    van den Brule F, Berndt S, Simon N, et al. Trophoblast invasion and placentation: molecular mechanisms and regulation. Chem Immunol Allergy. 2005;88:163–180.

  14. 14.

    Cao W, Henry MD, Borrow P, et al. Identification of alpha-dystroglycan as a receptor for lymphocytic choriomeningitis virus and Lassa fever virus. Science. 1998;282(5396):2079–2081.

  15. 15.

    Barton LL, Mets MB, Beauchamp CL. Lymphocytic choriomeningitis virus: emerging fetal teratogen. Am J Obstet Gynecol. 2002;187(6):1715–1716.

  16. 16.

    Saito F, Saito-Arai Y, Nakamura A, Shimizu T, Matsumura K. Processing and secretion of the N-terminal domain of alpha-dystroglycan in cell culture media. FEBS Lett. 2008;582(3): 439–444.

  17. 17.

    Singh J, Itahana Y, Knight-Krajewski S, et al. Proteolytic enzymes and altered glycosylation modulate dystroglycan function in carcinoma cells. Cancer Res. 2004;64(17):6152–6159.

  18. 18.

    Losasso C, Di Tommaso F, Sgambato A, et al. Anomalous dystroglycan in carcinoma cell lines. FEBS Lett. 2000; 484(3):194–198.

  19. 19.

    Qiu Q, Yang M, Tsang BK, Gruslin A. EGF-induced trophoblast secretion of MMP-9 and TIMP-1 involves activation of both PI3K and MAPK signalling pathways. Reproduction. 2004;128(3): 355–363.

  20. 20.

    Bozzi M, Inzitari R, Sbardell D, et al. Enzymatic processing of beta-dystroglycan recombinant ectodomain by MMP-9: identification of the main cleavage site. IUBMB Life. 2009;61(12): 1143–1152.

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Correspondence to Reagan M. Street MD.

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Street, R.M., Mucowski, S.J., Urrabaz-Garza, R. et al. Dystroglycan Expression in Human Placenta: Basement Membrane Localization and Subunit Distribution Change Between the First and Third Trimester. Reprod. Sci. 19, 282–289 (2012). https://doi.org/10.1177/1933719111419247

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Keywords

  • dystroglycan
  • placenta
  • trophoblast