Recently, boldenone (androgenic steroid) is used in improvement of the growth and food conversion in food-producing animals. In addition, it is used by bodybuilders during both off-season and precontest, where it is well known for increasing vascularity while preparing for a bodybuilding contest. The present study was designed to investigate the possible effect of growth promoter boldenone undecylenate on the structure and functions of rabbit testes. A total of 32 adult New Zealand rabbits were divided into 4 groups. The first group in the control group includes animals that were intramuscularly injected with olive oil and dissected after 3 weeks. Three experimental groups include animals that receive 1, 2, and 3 intramuscular injections of 5 mg/kg body weight boldenone, and dissected after 3, 6, and 9 weeks, respectively. Treating rabbits with boldenone increased the testosterone levels compared to the control group. Seminiferous tubules of the rabbit testis treated with boldenone showed reduced development and degeneration of the germinal epithelium, leading to debris and syncytial cell formation in the lumina of seminiferous tubules. Our immunohistochemical results indicated severe reduction in proliferating cell nuclear antigen–positive spermatogonia in boldenone-treated animals as compared to the control group. These findings explain the common phenomena among athletics and bodybuilders who suffer from infertility as they were injected with some drugs such as steroids (boldenone) to build muscles.
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Hughes TK, Fulep E, Juelich T, Smith EM, Stanton GJ. Modulation of immune responses by anabolic-androgenic steroids. Int J Immunopharmacol. 1995;17(11):857–863.
Sullivan ML, Martinez CM, Gennis P, Gallagher EJ. The cardiac toxicity of anabolic steroids. Prog Cardiovasc Dis. 1998;41(1): 1–15.
Pey A, Blázquez I, Delgad J, Megýas A. Effects of prolonged stanozolol treatment on antioxidant enzyme activities, oxidative stress markers, and heat shock protein HSP72 levels in rat liver. J Steroid Biochem Mol Biol. 2003;87(4–5):269–277.
Schänzer W. Metabolism of anabolic-androgenic steroids. Clin Chem. 1996;42(7): 1001–1020.
Sundlof SF.Legal control of veterinary drugs. In: Adams HR, ed. Veterinary Pharmacology Therapeutics. 8th ed. Ames, IA: Iowa State University Press; 2001:1155.
Soma LR, Uboh CE, Guan F, Mc-Donnell S, Pack J. Pharmacokinetics of boldenone and stanozolol and the results of quantification of anabolic-androgenic steroids in race horses and nonrace horses. J Vet Pharmacol Ther. 2007;30(2):101–108.
Kicman AT. Pharmacology of anabolic steroids. Br J Pharmacol. 2008;154(3):502.
Guan F, Cornelius EU, Soma R, Youwen Y, Liu Y, Lia X. High-throughput UHPLC MS/MS method for the detection, quantification and identification of fifty-five anabolic-androgenic steroids in equine plasma. J Mass Spectrom. 2010;45(11):1270–1279.
Kuhn CM. Anabolic steroids. Recent Prog Horm Res. 2002;57: 411–434.
Cannizzo TF, Zancanaro G, Spada F, Mulasso C, Biolatti B. Pathology of the testicle and sex accessory glands following the administration of boldenone and boldione as growth promoters in veal calves. J Vet Med Sci. 2007;69(11):1109–1116.
Hoffmann U. Anabolic steroids—a problem in popular sports. T + K. 2002;69(3):136.
Brookhouse B. Two more positive drug tests. Fight World. bloodyelbow.com. http://www.bloodyelbow.com/story/2007/8/17/02615/9740.
Gabr F, Abo El-Maaty T, Amal M, Aotifa AM. Effects of growth promoter boldenone undecylenate on weaned male lambs. Nature Sci. 2009;7(3):61–69.
Tietz NW.Textbook of Clinical Chemistry. In: Burtis CA, Ashwood ER. eds. 2nd ed. London, UK: W.B. Saunders Co; 1994.
Bancroft JD, Cook HC. Immunocytochemistry. In Manual of Histological Techniques and their Diagnostic Applications, Bancroft JD and Cook HC (eds) pp. 263–288. Churchill Livingstone: Edinburgh.
Tousson E, Ali EM, Ibrahim W, Mansour A. PCNA as a molecular biomarker for spermatogenesis in PTU-induced hypothyroidism of rats. Reprod Sci. 2011;18(7):679–686.
Urhausen A, Torsten A, Wilfried K. Reversibility of the effects on blood cells, lipids, liver function and hormones in former anabolic-androgenic steroid abusers. J Steroid Biochem Mol Biol. 2003;84(2–3):369–375.
Takahashi M, Tatsugi Y, Kohno T. Endocrinological and pathological effects of anabolic-androgenic steroid in male rats. Endocr J. 2004;51(4):425–434.
Gabr FI, Shaker MH. Effect of anabolic steroids (boldenone undecylenate) on metabolic, hormones and reproductive performance of mature rams. . J Egypt Vet Med Assoc. 2006; 66(2):235–248.
Simontacchi C, Perez de Altamirano T, Marinelli L, Angeletti R, Gabai G. Plasma steroid variations in bull calves repeatedly treated with testosterone, nortestosterone and oestradiol administered alone or in combination. Vet Res Commun. 2004;28(6): 467–477.
Shimomura K, Shimada M, Hagiwara M, Harada S, Kato M, Furuhama K. Insights into testicular damage induced by ethinylestradiol in rats. Reprod Toxicol. 2005;20(1):157–163.
Groot MJ, Biolatti B. Histopathological effects of boldenone in cattle. J Vet Med. 2004;51(2):58–63.
Veeramachaneni DN, Sherman GB, Floyd JG, Ott RS, Hixon JE. Zeranol and estradiol induce similar lesions in the testes and epididymides of the prepubertal beef bull. Fund Appl Toxicol. 1988; 10(1):73–81.
Silcox RW, Keeton JT, Johnson BH. Effects of zeranol and trenbolone acetate on testis function, live weight gain and carcass traits of bulls. J Anim Sci. 1986;63(2):358–368.
Rodriguez BM, Mendez Sanchez A, Blanco Rodriguez A. Comparative morphological studies of lamb and calf Sertoli cells treated with anabolic agent. Histol Histopathol. 1991;6(2): 171–174.
Groot MJ, Arts CJM. Histological changes in the genital tract of male veal calves after the administration of natural hormones. Arch Lebensmittelhyg. 1991;42:93–96.
Yesalis CE, Kennedy NJ, Kopstein AN, Bahrke MS. Anabolicandrogenic steroid use in the United States. J Am Vet Med Assoc. 1993;270(10):1217–1221.
Madsen P, Celis JE. S-Phase patterns of cyclin (PCNA) antigen staining resemble topographical patterns of DNA synthesis. FEBS Lett. 1995;193(1):5–11.
Zeng L, Kong XT, Su JW, Xia TL, Na YQ, Guo YL. Evaluation of germ-cell kinetics in infertile patients with proliferating cell nuclear antigen proliferating index. Asian J Androl. 2001;3(1): 63–66.
Toshihiro I. Thyroid hormone and atherosclerosis. Vasc Pharmacol. 2010 Mar-Apr;52(3–4):151–156.
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Tousson, E., El-Moghazy, M., Massoud, A. et al. Histopathological and Immunohistochemical Changes in the Testes of Rabbits After Injection With the Growth Promoter Boldenone. Reprod. Sci. 19, 253–259 (2012) doi:10.1177/1933719111418126