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Endocrine Disruptors, Polychlorinated Biphenyls-Induced gC1qR-dependent Apoptosis in Human Trophoblast Cell Line HTR-8/SVneo

Abstract

Although an association exists between exposure to polychlorinated biphenyls (PCBs) and spontaneous miscarriage, the mechanisms underlying this phenomenon remain unclear. In this study, PCBs content in plasma was detected by gas chromatography–mass spectrometry (GC-MS) and decidua tissues were examined for the expression of globular heads of C1q receptor (gC1qR) using Western blot in patients who underwent induced abortion and spontaneous abortion. Results showed increased PCBs content and gC1qR expression in patients who experienced spontaneous abortion. In vitro, Western blot analysis demonstrated significantly higher caspase 3 expression and apoptotic cell counts in green fluorescent protein (GFP)-gC1qR vector group. Additionally, gC1qR and caspase 3 showed decreased expression following PCBs plus gC1qR small interfering RNA (siRNA) treatment. The percentage of apoptotic cells increased in cells treated with PCBs alone or PCB plus negative siRNA. These data suggest that maternal exposure to PCBs is associated with adverse pregnancy outcomes and that upregulation of gC1qR is important for PCBs-mediated trophoblast cell apoptosis.

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References

  1. 1.

    Vaithinathan S, Saradha B, Mathur PP. Methoxychlor induces apoptosis via mitochondria-and FasL-mediated pathways in adult rat testis. Chem Biol Interact. 2010;185(2):110–118.

  2. 2.

    Eskenazi B, Mocarelli P, Warner M, et al. Maternal serum dioxin level and birth outcomes in women of Seveso, Italy. Environ Health Perspect. 2003;111(7):947–953.

  3. 3.

    Murono EP, Derk RC, Akgul Y. In vivo exposure of young adult male rats to methoxychlor reduces serum testosterone levels and ex vivo Leydig cell testosterone formation and cholesterol side-chain cleavage activity. Reprod Toxicol. 2006;21(2):148–153.

  4. 4.

    Martinez EM, Swartz WJ. Effects ofmethoxychlor on the reproductive system of the adult female mouse: 2. Ultrastructural observations. Reprod Toxicol. 1992;6(1):93–98.

  5. 5.

    Swartz WJ, Corkern M. Effects of methoxychlor treatment of pregnant mice on female offspring of the treated and subsequent pregnancies. Reprod Toxicol. 1992;6(5):431–437.

  6. 6.

    Wang Q, Zhao XF, Ji YL, et al. Mitochondrial signaling pathway is also involved in bisphenol A induced germ cell apoptosis in testes. Toxicol Lett. 2010;199(2):129–135.

  7. 7.

    Wang T, Wang Y, Fu J, et al. Characteristic accumulation and soil penetration of polychlorinated biphenyls and polybrominated diphenyl ethers in wastewater irrigated farmlands. Chemosphere. 2010;81(8):1045–1051.

  8. 8.

    Storelli MM, Barone G, Storelli A, Marcotrigiano GO. Levels and congener profiles of PCBs and PCDD/Fs in blue shark (Prionace glauca) liver from the South-Eastern Mediterranean Sea (Italy). Chemosphere. 2011;82(1):37–42.

  9. 9.

    Revich B, Aksel E, Ushakova T, et al. Dioxin exposure and public health in Chapaevsk, Russia. Chemosphere. 2001;43(4–7):951–966.

  10. 10.

    Kozul D, Romanic SH. Level and distribution of PCBs and organochlorine pesticides in the air, pine needles, and human milk. Arh Hig Rada Toksikol. 2010;61(3):339–356.

  11. 11.

    Muta T, Kang D, Kitajima S, Fujiwara T, Hamasaki N. P32 protein, a splicing factor 2-associated protein, is localized in mitochondrial matrix and is functionally important in maintaining oxidative phosphorylation. J Biol Chem. 1997;272(39): 24363–24370.

  12. 12.

    Rubinstein DB, Stortchevoi A, Boosalis M, et al. Receptor for the globular heads of C1q (gC1q-R; p33; Hyaluronan-binding protein is preferentially expressed by adenocarcinoma cells. Int Cancer Res. 2004;110(5):741–750.

  13. 13.

    Peerschke EI, Petrovan RJ, Ghebrehiwet B, Ruf W. Tissue factor pathway inhibitor-2 (TFPI-2) recognizes the complement and kininogen binding protein gC1qR/p33 (gC1qR): implications for vascular inflammation. Thromb Haemost. 2004;92(4):811–819.

  14. 14.

    Xu L, Xiao N, Liu F, Ren H, Gu J. Inhibition of RIG-I and MDA5-dependent antiviral response by gC1qR at mitochondria. Proc Natl Acad Sci USA. 2009;106(5):1530–1535.

  15. 15.

    Meenakshi J, Anupama SK, Datta K. Constitutive expression of hyaluronan binding protein 1 (HABP1/p32/gC1qR) in normal fibroblast cells perturbs its growth characteristics and induces apoptosis. Biochem Biophys Res Commun. 2003;300(3):686–693.

  16. 16.

    Croley TR, Hughes RJ, Koenig BG, Metcalfe CD, March RE. Mass spectrometry applied to the analysis of estrogens in the environment, Rapid Commun. Mass Spectrom. 2000;14(13): 1087–1093.

  17. 17.

    Small CM, Cheslack-Postava K, Terrell M, et al. Risk of spontaneous abortion among women exposed to polybrominated biphenyls. Environ Res. 2007;105(2):247–255.

  18. 18.

    Glaser SL, Clarke CA, Keegan TH, et al. Attenuation of social class and reproductive risk factor associations for Hodgkin lymphoma due to selection bias in controls. Cancer Causes Control. 2004;15(7):731–739.

  19. 19.

    Bulletti C, Flamigni C, Giacomucci E. Reproductive failure due to spontaneous abortion and recurrent miscarriage. Hum Reprod Update. 1996;2(2):118–136.

  20. 20.

    Law DC, Klebanoff MA, Brock JW, Dunson DB, Longnecker MP. Maternal serum levels of polychlorinated biphenyls and 1, 1-dichloro-2, 2-bis (p-chlorophenyl) ethylene (DDE) and time to pregnancy. Am J Epidemiol. 2005;162(6):523–532.

  21. 21.

    Axmon A, Rylander L, Strömberg U, Jönsson B, Nilsson-Ehle P, Hagmar L. Polychlorinated biphenyls in serum and time to pregnancy. Environ Res. 2004;96(2):186–195.

  22. 22.

    Mallick J, Datta K. HABP1/p32/gC1qR induces aberrant growth and morphology in Schizosaccharomyces pombe through its N-terminal alpha helix. Exp Cell Res. 2005;309(2):250–263.

  23. 23.

    Peerschke EI, Murphy TK, Ghebrehiwet B. Activation-dependent surface expression of gC1qR/p33 on human blood platelets. Thromb Haemost. 2003;89(2):331–339.

  24. 24.

    Peerschke EI, Ghebrehiwet B. The contribution of gC1qR/p33 in infection and inflammation. Immunobiology. 2007;212(4–5):333–342.

  25. 25.

    Fogal V, Richardson AD, Karmali PP, Scheffler IE, Smith JW, Ruoslahti E. Mitochondrial p32 protein is a critical regulator of tumor metabolism via maintenance of oxidative phosphorylation. Mol Cell Biol. 2010;30(6):1303–1318.

  26. 26.

    Peerschke EI, Minta JO, Zhou SZ, et al. Expression of gC1q-R/p33 and its major ligands in human atherosclerotic lesions. Mol Immunol. 2004;41(8):759–766.

  27. 27.

    Vandivier RW, Ogden CA, Fadok VA, et al. Role of surfactant proteins A, D, and C1q in the clearance of apoptotic cells in vivo and in vitro: calreticulin and CD91 as a common collectin receptor complex. J Immunol. 2002;169(7):3978–3986.

  28. 28.

    Chowdhury AR, Ghosh I, Datta K. Excessive reactive oxygen species induces apoptosis in fibroblasts: role of mitochondrially accumulated hyaluronic acid binding protein 1 (HABP1/p32/ gC1qR). Exp Cell Res. 2008;314(3):651–667.

  29. 29.

    Fogal V, Zhang L, Krajewski S, Ruoslahti E. Mitochondrial/ cell-surface protein p32/gC1qR as a molecular target in tumor cells and tumor stroma. Cancer Res. 2008;68(17):7210–7218.

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Correspondence to Yu-Zhu Peng PhD.

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Gu, P., Gao, L., Li, L. et al. Endocrine Disruptors, Polychlorinated Biphenyls-Induced gC1qR-dependent Apoptosis in Human Trophoblast Cell Line HTR-8/SVneo. Reprod. Sci. 19, 181–189 (2012) doi:10.1177/1933719111415866

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Keywords

  • polychlorinated biphenyls (PCBs)
  • globular heads of C1q receptor (gC1qR)
  • apoptosis