Reproductive Sciences

, Volume 19, Issue 1, pp 55–63 | Cite as

Temporal Investigation of Matrix Metalloproteinases and Their Inhibitors in Human Cervicovaginal Fluid in Late Pregnancy and Labor

  • Yujing J. Heng
  • Megan K. W. Di Quinzio
  • Stella Liong
  • Michael Permezel
  • Gregory E. Rice
  • Harry M. GeorgiouEmail author
Original Articles


Temporal expression of matrix metalloproteinase (MMP)-1, -2, -3, -7, -8, -9, -12, and -13, and tissue inhibitors of metalloproteinases (TIMPs)-1 and -2 in human cervicovaginal fluid (CVF) in term pregnancy and labor was investigated. Term parous women provided CVF samples that were grouped into labor, 1 to 3, 6 to 8, and 12 to 16 days before labor onset. Both MMPs and TIMPs (n = 60) were quantified using multiplex solution array and enzyme-linked immunosorbent assays, respectively. Further analysis of TIMP-1 (n = 180) was undertaken. All MMPs and TIMPs except MMP-12 and -13 were detected in the CVF. Matrix metalloproteinase 7, TIMP-1, and TIMP-2 were significantly increased in labor. Tissue inhibitors of metalloproteinase 1 was significantly increased up to 7 days before spontaneous labor onset. The data suggest a role of MMP-7 in the remodeling and rupture of fetal membranes and may reflect the homeostatic regulation of extracellular matrix remodeling of MMP-7 by TIMP-1 and TIMP-2.


cervicovaginal fluid labor matrix metalloproteinases pregnancy tissue inhibitors of matrix metalloproteinases 


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Copyright information

© Society for Reproductive Investigation 2012

Authors and Affiliations

  • Yujing J. Heng
    • 1
    • 2
  • Megan K. W. Di Quinzio
    • 1
    • 2
  • Stella Liong
    • 1
    • 2
  • Michael Permezel
    • 1
    • 2
  • Gregory E. Rice
    • 1
    • 3
  • Harry M. Georgiou
    • 1
    • 2
    • 4
    Email author
  1. 1.Department of Obstetrics and GynaecologyThe University of MelbourneParkvilleAustralia
  2. 2.Mercy Perinatal Research CentreMercy Hospital for WomenHeidelbergAustralia
  3. 3.University of Queensland Centre for Clinical ResearchHerstonAustralia
  4. 4.University Department, Mercy Hospital for WomenHeidelbergAustralia

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