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Hormonal Regulation of Galectin 3 in Trophoblasts and Its Effects on Endometrium

Abstract

Previous studies had shown important functions of galectin 3 (Gal-3) in endometrium during embryo implantation, in regulation of endometrial cell proliferation and adhesion by interacting with integrin β3. In this study, we investigated hormonal regulation of Gal-3 in trophoblasts and its extracellular effects on endometrium. We used BeWo and RL95-2 cells as a model of trophoblastic and endometrial epithelial cells, respectively, to create an in vivo model of embryo implantation. Our results indicated that 17β-estradiol (E2), progesterone (P4), and human chorionic gonadotropin (hCG) induced the expression of Gal-3 and promoted its secretion from BeWo cells. The exogenous Gal-3 inhibited cell proliferation and induced apoptosis of endometrial cells (RL95-2 cells) through activation of integrin β1. We further validated the proapoptotic effect of Gal-3 secreted by trophoblastic cell on endometrial cells by culturing RL95-2 cells with Bewo cells and measuring the apoptotic rate. Our analysis provides new insight into the critical roles of Gal-3 in embryo implantation.

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Correspondence to Wei Zhang MD, PhD.

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Yang, H., Taylor, H.S., Lei, C. et al. Hormonal Regulation of Galectin 3 in Trophoblasts and Its Effects on Endometrium. Reprod. Sci. 18, 1118–1127 (2011) doi:10.1177/1933719111407212

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Keywords

  • galectin 3
  • estrogen
  • progesterone
  • human chorionic gonadotropin
  • integrin β1