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Research on Chemical Intermediates

, Volume 22, Issue 2, pp 129–136 | Cite as

Chlorophyllin coupled with polyethylene glycol: A potent photosensitizer

  • T. Itoh
  • A. Ishii
  • Y. Kodera
  • M. Hiroto
  • A. Matsushima
  • H. Nishimura
  • Y. Inada
Article

Abstract

Chlorophyllin a prepared by the saponification of chlorophyll a was conjugated with α-(3-aminopropyl)-ω-methoxypoly(oxyethylene), PEG-NH2, to form PEG-chlorophyllin through acid-amide bonds. PEG-chlorophyllin was readily soluble not only in aqueous solution but also in organic solvents. The absorption maxima of the conjugate were located at 657 nm and 418 nm in aqueous solution, and at 656 nm and 422 nm in benzene. The conjugate seems to be more stable toward light-illumination than uncoupled chlorophyllin. Furthermore, PEG-chlorophyllin possessed a potent photosensitizing activity to produce superoxide anion during the illumination, although chlorophyllin immediately lost its activity by light-illumination.

Keywords

Polyethylene Glycol Nitro Blue Tetrazolium Produce Superoxide Anion Spirulina Chlorophyllin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer 1996

Authors and Affiliations

  • T. Itoh
    • 1
  • A. Ishii
    • 1
  • Y. Kodera
    • 1
  • M. Hiroto
    • 1
  • A. Matsushima
    • 1
  • H. Nishimura
    • 1
  • Y. Inada
    • 1
  1. 1.Toin Human Science and Technology Centre, Department of Materials Science and TechnologyToin University of YokohamaYokohamaJapan

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