Research on Chemical Intermediates

, Volume 33, Issue 1–2, pp 79–90 | Cite as

Amplified fluorescence quenching and biosensor application of a poly (para-phenylene) cationic polyelectrolyte

  • Mauricio R. Pinto
  • Chunyan Tan
  • Michael B. Ramey
  • John R. Reynolds
  • Troy S. Bergstedt
  • David G. Whitten
  • Kirk S. SchanzeEmail author


The quenching behavior of a water-soluble cationic poly (para-phenylene) bearing quaternized ammonium side groups (P-NEt 3 + ) was studied. P-NEt 3 + is efficiently quenched by sodium anthraquinone-2,6-disulfonate (AQS) and sodium 1,4,5,8-naphthalenediimide-N,N’-bis (methylsulfonate) (NDS) in aqueous solution via a photo-induced electron-transfer mechanism. Absorption spectra of the NDS/P-NEt 3 + ion-pair complex indicated formation of a stable charge-transfer complex in the ground state. A large spectral shift and band broadening occurred during AQS/P-NEt 3 + complex formation, which is believed to arise due to P-NEt 3 + conformational changes induced by hydrophobic interactions. Finally, a protein sensor that relies on the quenching behavior of P-NEt 3 + was designed based on the quencher-tether-ligand (QTL) approach. AQS tethered to biotin (AQS-E-Biotin) was used along with P-NEt 3 + to sense avidin.


Quencher-tether-ligand (QTL) approach quenching biosensor poly(para-phenylene) polyelectrolyte 


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Copyright information

© Springer 2007

Authors and Affiliations

  • Mauricio R. Pinto
    • 1
  • Chunyan Tan
    • 1
  • Michael B. Ramey
    • 1
  • John R. Reynolds
    • 1
  • Troy S. Bergstedt
    • 2
  • David G. Whitten
    • 2
  • Kirk S. Schanze
    • 1
    Email author
  1. 1.Department of ChemistryUniversity of FloridaGainesvilleUSA
  2. 2.QTL BiosystemsLLCSanta FeUSA

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